福建省鼠伤寒沙门菌PFGE分子分型和耐药的关联性研究

目的 分析福建省鼠伤寒沙门菌的脉冲场凝胶电泳（PFGE）分子分型和耐药结果之间的关联性，探讨二者的内在联系。 方法 对福建省66株鼠伤寒沙门菌进行PFGE分子分型和药敏试验，并对分子分型和药敏试验结果进行关联性分析。 结果 66株鼠伤寒沙门菌分为32个PFGE型，P1、P8型为优势型别，其中40.9%（27/66）的菌株为P1型，10.6%（7/66）的菌株为P8型， 51.52%（34/66）的试验菌株归于P1和P8型别；耐药结果经聚类分析，可将抗生素分为4类，其中头孢类和环丙沙星（氟喹诺酮类）是较高敏感药物，敏感率分别为94.42%（61/66）和62.12%（41/66）；P1型在多重耐药严重的菌株中（>6耐）为优势型别，占该类菌株数的56.82%，以6耐为分界，P1型菌株在两组中的分布存在差异，并具有统计学意义；P8型菌株在两组中的分布差异无统计学意义。结论 P1和P8型是福建省鼠伤寒沙门菌的PFGE分型的优势基因型，其中P1型与多重耐药严重的菌株（>6耐）间存在着关联性；头孢类和环丙沙星（氟喹诺酮类）仍是当前治疗福建省严重耐药鼠伤寒菌的主要药物。

Association between PFGE molecular typing and drug resistance of Salmonella typhimurium in Fujian

ABSTRACT: To study the association between PFGE molecular typing and drug resistance of Salmonella typhimurium in Fujian, we carried out PFGE experiment and susceptibility test within 66 strains of S. typhimuriumn, and conducted association analysis. The 66 S. typhimurium were divided into 32 PFGE types. And as 40.9%（27/66）were P1, and 10.6%（7/66）were P8, there were 51.52%（34/66）strains classified into P1 and P8, which were the prevalent types. In the drug resistance test, antibiotics were divided into 4 groups with cluster analysis, in which, cephalosporin and ciprofloxacin (quinolone ) were hypersensitive, and the sensitive rates were 94.42%（61/66）and 62.12%（41/66） respectively；P1 was the prevalent type in multidrug resistant strains (more than 6 resistant), amounting to 56.82% of the total. The distribution of P1 had statistical significance between the group “more than 6 strains resistance” and the group “less than 6 strains resistance”, while the distribution of P8 had no statistical significance between these two groups. Therefore, P1 and P8 were the prevalent gene types, and there was relationship between P1 and multidrug resistant strains (more than 6 strains resistance). At present, cephalosporin and ciprofloxacin (quinolone) are still the main antibiotics to cure Salmonella typhimurium in Fujian.