尾吊影响大鼠比目鱼肌Dystrophin表达分布以及血清乳酸脱氢酶活性***◆

背景：抗肌萎缩蛋白Dystrophin在肌肉运动性损伤时易发生改变。 目的：观察模拟失重效应对大鼠比目鱼肌抗肌萎缩蛋白Dystrophin表达、分布及血清乳酸脱氢酶活性的影响。 方法：采用大鼠后肢尾吊模拟失重效应模型,分别在尾吊1,4,7,10,14 d分离SD大鼠比目鱼肌并提取血清进行检测。 结果与结论：随着尾吊时间的延长,大鼠肌纤维横截面积减小;肌膜上Dystrophin呈现弥散性分布的趋势,甚至出现Dystrophin断裂现象;Dystrophin mRNA表达下降。同时尾吊7 d,大鼠血清乳酸脱氢酶活性升高。提示失重引起的肌萎缩,伴随着Dystrophin mRNA的表达下降和蛋白分布弥散性变化,而乳酸脱氢酶活性变化提示失重性肌萎缩可能与肌损伤的发生有关。关键词：肌萎缩;失重;比目鱼肌;Dystrophin;乳酸脱氢酶 doi:10.3969/j.issn.1673-8225.2012.15.036

Hindlimb suspension affects the expression and distribution of Dystrophin in rat soleus and serum lactate dehydrogenase activity

BACKGROUND: Dystrophin is the most prone to change in sports injuries.  OBJECTIVE: To investigate the effects of simulated weightlessness on the Dystrophin expression and distribution in the soleus muscle, as well as the activity changes of lactate dehydrogenase in serum. METHODS: A rat hindlimb suspension model was used to simulate weightlessness effect. After 1, 4, 7, 10 and 14 days of hindlimb suspension, the soleus and serum were isolated from SD rats for parameter examination. RESULTS AND CONCLUSION: With the prolonged hindlimb suspension, muscle fiber cross-sectional area decreased and immunofluorescence results showed that Dystrophin expression exhibited a diffuse distribution trend, even appeared with disrupted distribution on the sarcolemma. Quantitative PCR results showed that the hindlimb suspension resulted in reduced expression of Dystrophin mRNA. Lactate dehydrogenase activity during 7-day hindlimb suspension appeared with a significant increase. Muscle atrophy induced by hindlimb suspension is accompanied with diffusion of Dystrophin distribution and decreased expression of Dystrophin mRNA. Activity changes of lactate dehydrogenase indicate that muscular atrophy may be associated with muscle injury.