壳聚糖/磷酸三钙复合组织工程支架材料的制备及其性能*

背景：通过适当的工艺混合、加工来制备复合支架材料,可以弥补单一材料的不足,最大限度地满足组织工程的需要。 目的：制备壳聚糖/磷酸三钙复合支架,探讨其作为牙髓组织工程支架材料的可行性。 方法：壳聚糖粉末溶于微量冰醋酸溶液中,搅拌均匀,静置脱泡,预冷冻,交联,再次冷冻制成海绵状多孔壳聚糖/磷酸三钙支架。 结果与结论：冻干法制备的壳聚糖/磷酸三钙多孔支架平均孔隙率达85.78%,最高孔隙率达90%以上,孔径在100~300 μm,复合后的支架材料具有良好的韧性,当轴向压缩变形量超过5 mm时,材料仍然没有发生破坏。材料浸提液与牙髓细胞复合培养后,细胞毒性均为0级,由此可见壳聚糖/磷酸三钙复合材料具有良好的生物相容性、细胞亲和性和一定的力学性能,满足生物材料基本要求。

Preparation and properties of chitosan/tricalcium phosphate composite scaffolds

BACKGROUND: Composite scaffolds prepared with appropriate mixing process can make up the insufficient of single material and satisfy the needs of tissue engineering in maximum. OBJECTIVE: To prepare the chitosan/tricalcium phosphate composite scaffold and explore its feasibility of serving as scaffold for pulp tissue engineering. METHODS: Chitosan powder was dissolved in small amount of acetic acid solution. The mixture was stirred to uniform, stilled to deaeration. Then pre-cooling and cross-linking were performed. Sponge-like chitosan/tricalcium phosphate composite scaffold was prepared after anther pre-cooling and cross-linking. RESULTS AND CONCLUSION: Average porosity of the chitosan/tricalcium phosphate composite scaffold prepared by freeze-drying method was 85.78% and the highest porosity was over 90%. The aperture was 100-300 μm. The composite scaffold had a good toughness, and could not be damaged at over 5 mm of axial compression deformation. The cell toxicity was below grade 0 after cultured in the extracts of the materials. The chitosan/tricalcium phosphate composite scaffold can satisfy the basic needs of biomaterials based on its good biocompatibility, cell affinity, and some mechanical properties.