MMP-1和TFPI-2在鼻咽癌中的表达及意义

探讨鼻咽癌(NPC)组织中基质金属蛋白酶1(MMP-1)和组织因子途径抑制因子2(TFPI-2)的表达在NPC侵袭转移中的意义。方法应用免疫组织化学MaxVision法检测50例NPC组织(NPC组)和20例慢性鼻咽炎黏膜组织(对照组)中MMP-1和TFPI-2的表达,并分析其与患者临床参数的关系。结果 NPC组MMP-1的阳性表达率为78%,高于对照组的35%(P<0.05);而TFPI-2在NPC组的阳性表达率为20%,低于对照组的85%(P<0.05)。NPC组伴有淋巴结转移的癌组织MMP-1表达水平高于无淋巴结转移的癌组织(P<0.05),而伴有淋巴结转移的癌组织TFPI-2表达水平低于无淋巴结转移的癌组织(P<0.05),且MMP-1与TFPI-2的表达呈负相关(r=-0.490,P<0.05)。结论 MMP-1的高表达和TFPI-2的低表达与NPC颈淋巴结转移关系密切,两者表达平衡失调可能是NPC高转移的重要因素,可作为评估NPC转移的生物指标。

Expression of Matrix Metalloproteinase-1(MMP-1) and Tissue Factor Pathway Inhibitor-2(TFPI-2) in Nasopharyngeal Carcinoma and Its significance

Objective To investigate the expression of matrix metalloproteinase-1(MMP-1) and tissue factor pathway inhibitor-2(TFPI-2) in nasopharyngeal carcinoma(NPC),and to explore its correlation with the invasion and metastasis of NPC.Methods Immunohistochemical staining(MaxVision) was used to detect the expression of MMP-1 and TFPI-2 in 50 NPC tissues and 20 chronic nasopharyngitis tissues,and the relationship of MMP-1 and TFPI-2 expression to pathological and clinical features of NPC were statistically analyzed.Results Compared with chronic nasopharyngitis tissues,MMP-1 positive rate increased but TFPI-2 positive rate decreased in NPC tissues(78% vs 35% and 20% vs 85%,respectively;P<0.05).Furthermore,the higher expression of MMP-1 and lower expression of TFPI-2 were found in NPC tissues with cervical lymph node metastasis than those in NPC without lymph node metastasis(all P<0.05),and MMP-1 expression was negatively correlated with TFPI-2 expression(r=-0.490,P<0.05).Conclusion The higher expression of MMP-1 and lower expression of TFPI-2 are closely related to NPC with cervical lymph node metastasis.The imbalance of MMP-1 and TFPI-2 expression is an important factor inducing high metastasis of NPC,and may be used as a biological indicator in the metastatic assessment of NPC.