胰岛素样生长因子1及血小板源性生长因子对人退变髓核细胞生物学活性的影响

背景：有研究表明胰岛素样生长因子1和血小板源性生长因子可抑制人椎间盘细胞凋亡。目的：观察在体外培养条件下胰岛素样生长因子1、血小板源性生长因子对人退变髓核细胞生物学活性的影响。方法：体外单层培养人退变髓核细胞,通过免疫组织化学鉴定细胞。对传3代人退变髓核细胞采用分别不同生长因子干预,实验分4组：胰岛素样生长因子1组,血小板源性生长因子组,胰岛素样生长因子1+血小板源性生长因子组及对照组。 结果与结论：胰岛素样生长因子1、血小板源性生长因子均可促进细胞增殖,促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,其中胰岛素样生长因子1促Ⅱ型胶原合成作用强于血小板源性生长因子(P < 0.05),血小板源性生长因子促蛋白聚糖合成作用强于胰岛素样生长因子1(P < 0.05)。胰岛素样生长因子1促进细胞合成Ⅰ型胶原,血小板源性生长因子抑制细胞合成Ⅰ型胶原。结果证实,胰岛素样生长因子1、血小板源性生长因子均可通过促进细胞增殖、促进细胞合成Ⅱ型胶原和聚集蛋白聚糖,从而提高人退变髓核细胞的生物学活性。

Effect of insulin-like growth factor-1 and platelet-derived growth factor on the biologicalpotential of human degenerative nucleus pulposus cells

BACKGROUND:Previous studies showed that insulin-like growth factor-1 (IGF-1) and platelet-derived growth factor (PDGF) cansuppress apoptosis of human intervertebral cells.OBJECTIVE:To observe the effects of IGF-1 and PDGF on the biological potential of the human degenerative NP cells in vitro.METHODS:Human degenerative nucleus pulposus cells were cultured in monolayer in vitro and identified byimmunohistochemical staining. The cells of passage 3 were treated separately with different growth factors and then randomlydivided into 4 groups: IGF-1 group, PDGF group, IGF-1+PDGF group and control group.RESULTS AND CONCLUSION:IGF-1 and PDGF could stimulate cell proliferation and synthesis of collagen type Ⅱ andaggrecan, and IGF-1 raised more content of collagen typeⅡ than the PDGF (P < 0.05), but the PDGF raised more content ofaggrecan than the IGF-1 (P < 0.05). IGF-1 stimulated the synthesis of collagen typeⅠ, then PDGF inhibited the synthesis. Boththe IGF-1 and PDGF can stimulate cell proliferation, the synthesis of collagen type Ⅱ and aggrecan, thereby enhance thebiological potential of the human degenerative nucleus pulposus cells.