脑出血大鼠急性期血浆及脑匀浆神经肽Y活性的变化

目的　观察大鼠脑出血急性期血浆及脑匀浆中神经肽 Y (NPY)活性的动态变化 ,探讨 NPY在脑出血脑损害中的作用。方法　 Wistar大鼠 70只 ,随机抽签法分为脑出血组及假手术对照组 ,每组各 35只 ;每组再按出血前及出血后 0 .5、6、12、2 4、4 8和 72 h分为 7个亚组 ,每亚组 5只。分别于各时间点测定血浆和血肿周边脑组织 NPY含量 ,并观察血肿病灶区的组织形态学病理改变。结果　脑出血后 6 h血浆和血肿周边脑组织 NPY的含量开始同步升高 ,并于 2 4 h达峰值 ,4 8h开始回落 ,但至 72 h时 NPY的含量仍显著高于出血前 (P<0 .0 5或 P<0 .0 1)。光镜和电镜下脑组织也发生相应的病理损害。结论　 NPY可能参与了脑出血的病理生理过程。

Changes of neuropeptide Y activity in plasma and brain tissue during intracerebral hemorrhage in rats

ObjectiveTo evaluate the significance of changes of neuropeptide Y(NPY) activity in plasma and brain tissue during experimental intracerebral hemorrhage(ICH). Methods: Seventy Wistar rats were randomly divided into two groups: control group and ICH group with each group subdivided into preoperation ,0.5 hours,6 hours,12 hours ,24 hours,48 hours and 72 hours postoperation subgroups, respectively (n=5). The ICH was established by infusing collagenase and heparin into rat caudate. The changes of NPY in plasma and perihemotoma at preoperation,0.5 hours,6 hours,12 hours,24 hours, 48 hours and 72 hours after operation were observed,respectively. NPY was determined by radio immunoassay . The morphologic change of brain was detected. Results: NPY activity in plasma and perihematoma increased synchronously after cerebral hemorrhage,and peaked at 24 hours,then began to reduce in 48 hours ,it was still higher than those of preoperation at 72 hours after hemorrhage(P<0.05 or P<0.01) . The correspondent pathological changes was observed in brain tissue under light microscope and electron microscope. Conclusion: NPY might be involved in the pathogenesis of cerebral hemorrhage.