胃良恶性病变组织中端粒酶的活性研究

目的:探讨胃癌和胃良性病变中端粒酶的活性及其临床意义.方法:应用TRAP-PCR-ELISA方法检测36例胃癌组织,20例胃溃疡组织,10例胃炎组织和20例正常人胃黏膜组织中端粒酶的活性.结果:胃癌组织中端粒酶阳性率分别为80.6%(29/36),胃良性病变组织中端粒酶阳性率为10%(其中2例胃溃疡和1例胃炎组织标本检测到端粒酶活性),正常人胃黏膜组织未检测到端粒酶阳性.胃癌组织与胃良性病变组织中端粒酶阳性率比较,差异有统计学意义(P＜0.01).结论:端粒酶活性是判断胃良恶性肿瘤的一个重要指标,对胃良性病变患者端粒酶检测阳性者应加强随访.

STUDY OF TELOMERASE ACTIVITY IN HUMAN BENIGN GASTRIC LESIONS AND GASTRIC CARCINOMAS

Objective:To study the telomerase expression in gastric carcinomas and benign gastric lesion and the clinical implications.Methods:Telomerase activity was detected by a TRAP-PCR-ELISA assay in tissue samples from 36 gastric carcinomas, 20 gastric ulcer, 10 gastritis, and 20 normal gastric muccosa. Result: The positive rate of telomerase activity was 80.6%(29/36) in the gastric cancerous tissue. Telomerase activity was positive in 10% of benign gastric lesion(2 gastric ulcer and 1 gastritis). NO telomerase activity was detected in normal gastric muccosa. Conclusion: Telomerase may be a good diagnostic marker for the diagnosis of gastric carcinomas and benign gastric lesion. It is suggested that followed-up activities for the telomerase positive case in benign gastric lesion patients are carried out.