| HPLC法测定罗库溴铵原料的含量 |
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| 引用本文： | 宋更申,周丽,姜建国,庞文哲,闫凯. HPLC法测定罗库溴铵原料的含量[J]. 国外医学(药学分册), 2010, 0(2): 127-129 |
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| 作者姓名： | 宋更申 周丽 姜建国 庞文哲 闫凯 |
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| 作者单位： | 河北省药品检验所;石家庄四药有限公司; |
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| 摘 要： | 目的建立高效液相色谱（HPLC）法测定罗库溴铵原料的含量。方法采用氨基键合硅胶为填充剂,以0.04mol/L四甲基氢氧化铵溶液（用磷酸调节pH值至7.4）-乙腈（10∶90）为流动相,柱温35℃,检测波长207nm。结果线性范围为0.1251～2.0065mg/ml（r^2=0.9999）;平均回收率为99.9%（RSD=0.1%）。结论采用HPLC法测定罗库溴铵原料的含量,方法灵敏度高,专属性强,结果准确可靠。
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| 关 键 词： | 高效液相色谱 罗库溴铵 含量测定 |
Determination of the content of rocuronium bromide by HPLC |
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| SONG Geng-shen,ZHOU Li,JIANG Jian-guo,PANG Wen-zhe,YAN Kai. Determination of the content of rocuronium bromide by HPLC[J]. Foreign Medical Sciences(Section of Pharmarcy), 2010, 0(2): 127-129 |
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| Authors: | SONG Geng-shen ZHOU Li JIANG Jian-guo PANG Wen-zhe YAN Kai |
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| Affiliation: | 1.Hebei Institute for Drug Control;Shijiazhuang 050011;China;2.Shijiazhuang No.4 Pharmaceutical Company Ltd;Shijiazhuang 050021;China |
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| Abstract: | Objective To establish a HPLC method to determinate the content of rocuronium bromide.Methods NH2-bond-ing silica gel was used as the stationary phase.The mobile phase consisted of 0.04 mol/L tetramethyl ammonium hydroxide（pH adjus-ted to 7.4 by phosphoric acid）and acetonitrile（10：90）.The column temperature was 35℃.The detection wavelength was 207 nm.Results The relation between concentration and peak area was linear in the range of 0.125 1-2.006 5 mg/ml with r^2 of 0.999 9.The average recovery rate was 99.9% with RSD of 0.1%.Conclusion This method is exclusive,sensitive and is reliable for the determi-nation of rocuronium bromide. |
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| Keywords: | HPLC rocuronium bromide content assay |
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