颅内动脉瘤患者血清蛋白组变化的研究

【目的】寻找颅内动脉瘤患者血清中表达差异的蛋白。【方法】采集12例血清蛋白样本(颅内动脉瘤患者4例、脑挫裂伤患者4例及正常成人本4例)用不同的CyDye染料交叉标记后依次进行双向胶内差异凝胶电泳(2-DDIGE)、图像分析及基质辅助激光解析离子化飞行时间质谱(MALDI-TOF-MS)鉴定。【结果】发现胶号为1119的蛋白质点,在颅内动脉瘤患者血清中比正常成人组表达升高1.82倍(P<0.05),而在脑挫裂伤患者血清中与正常成人的表达差异没有统计学意义(P>0.05)。该蛋白点经质谱鉴定为结合珠蛋白。【结论】结合珠蛋白在颅内动脉瘤患者血清中表达升高,可能参与了颅内动脉瘤的形成及扩张。

Serum Proteomic Variation Study of Patients with Intracranial Aneurysm

Objective To search differentially expressed proteins in serum of patient with intracranial aneurysm.Methods 12 Serum protein samples were recruited(4 cases of patients with intracranial aneurysm,4 cases of patients with brain laceration and 4 cases of natural adults),cross-labeled with variant CyDye,and then followed by two-dimensional differential in-gel electrophoresis(2-D DIGE),image analysis,and identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS).Results Spot 1119 was found highly expressed as 1.82 folds in serum of patients with intracranial aneurysm compared with that of normal adults(P < 0.05),while the differential expression of this protein between patients with brain laceration and normal adults was of no significance(P > 0.05).The protein was identified as haptoglobin using mass spectrometry.Conclusion Haptoglobin is highly expressed in patients with intracranial aneurysm,and probably contribute to formation and expansion of intracranial aneurysm.