Identification of B- and T-cell epitopes of BB,a carrier protein derived from the G protein of Streptococcus strain G148 |
| |
Authors: | Goetsch Liliane Haeuw Jean Francois Champion Thierry Lacheny Christine N'Guyen Thien Beck Alain Corvaia Nathalie |
| |
Institution: | Centre d'Immunologie Pierre Fabre, 74164 Saint-Julien en Genevois, France. |
| |
Abstract: | Most conventional vaccines consist of killed organisms or purified antigenic proteins. Such molecules are generally poorly immunogenic and need to be coupled to carrier proteins. We have identified a new carrier molecule, BB, derived from the G protein of Streptococcus strain G148. We show that BB is able to induce strong antibody responses when conjugated to peptides or polysaccharides. In order to localize T and B cell epitopes in BB and match them with the albumin-binding region of the molecule, we immunized mice with BB, performed B and T pepscan analyses, and compared the results with pepscan done with sera and cells from humans. Our results indicate that BB has two distinct T helper epitopes, seven linear B-cell epitopes, and one conformational B-cell epitope in BALB/c mice. Four linear B-cell epitopes were identified from human sera, three of which overlapped mouse B-cell epitopes. Finally, three human T-cell epitopes were detected on the BB protein. One of these T-cell epitopes is common to BALB/c mice and humans and was localized in the region that contains the albumin-binding site. These data are of interest for the optimization of new carrier molecules derived from BB. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|