JAK2-STAT3 / STAT5 信号通路与儿童食物过敏CD4+ T 淋巴细胞亚群变化的相关性研究

目的:探讨JAK2-STAT3/ STAT5 信号通路与儿童食物过敏CD4+ T 淋巴细胞亚群变化的相关性。方法:收集2015 年1 月至2017 年3 月来我院就诊的食物过敏患儿78 例,为过敏组;同期收集来我院体检的健康儿童78 例,为对照组。并根据儿童年龄将过敏组和对照组分为A、B、C、D 四组,其中A 组臆1 岁,B 组1 ~3 岁(不包括1 岁),C 组3 ~6 岁(不包括3 岁),D 组6 ~11 岁(不包括6 岁)。采用流式细胞仪检测CD4+ T 淋巴细胞亚群Th1、Th2、Th17 和调节性T 淋巴细胞(Treg)百分比,同时采用qPCR 检测外周血单核细胞JAK2、STAT3、STAT5 的mRNA 水平,分析两组儿童上述CD4+ T 淋巴细胞亚群和JAK2、STAT3、STAT5 表达水平的差异,并进一步分析JAK2-STAT3/ STAT5 信号通路和CD4+ T 淋巴细胞亚群的相关性。结果:对照组A 组、B 组、C 组儿童Th1、Treg 淋巴细胞百分比及Th1/ Th2、Treg/ Th17 比值均明显高于过敏组患儿A 组、B 组、C 组,而Th2、Th17 淋巴细胞百分比均小于过敏组所对应的同年龄段患儿,随儿童年龄增大,两组间差异均明显减小,但差异均有统计学意义(P<0.05)。对照组D 组儿童和过敏组D 组儿童上述指标差异均无统计学意义(P>0.05)。分别针对A、B、C 三组患儿JAK2、STAT3、STAT5 和CD4+ T 淋巴细胞亚群水平进行相关性比较,Th1、Treg、Th1/ Th2、Treg/ Th17 的相关性系数均<0(P<0.05),而Th2、Th17 的相关性系数均>0(P<0.05),且上述三组的相关系数绝对值逐次减小。上述信号通路分子与CD4+ T 淋巴细胞亚群水平在D 组均无明显相关性。结论:低龄食物过敏儿童的JAK2-STAT3/ STAT5 信号通路存在明显活化和CD4+ T淋巴细胞亚群改变现象,JAK2-STAT3/ STAT5 信号通路可有效调控CD4+ T 淋巴细胞亚群的分布,并伴随患儿年龄增大,上述作用相关性减弱并消失。

Correlation research between JAK2-STAT3 / STAT5 signal pathway and CD4+ T lymphocyte subsets in children with food allergy

Objective:To investigate the regulation of JAK2-STAT3/ STAT5 signaling pathway on CD4+ T lymphocyte subsets in children with food allergy.Methods:78 children with food allergy from January 2015 to March 2017 in our hospital were enrolled as the allergy group,and 78 healthy children in our hospital were enrolled as the control group.All the patients were divided into A,B,C,D groups according to ages.A group was less than or equal to 1 year old,B group of 1-3 years old(not including 1 year old),group C 3-6 years old(not including 3 years old),group D 6-11 years old(not including 6 years old).Flow cytometry was used to detect the CD4+ T lymphocyte subsets Th1,Th2,Th17 and regulatory T cells(Treg) and qPCR was used to detect the mRNA expression of peripheral blood mononuclear cells of JAK2,STAT3,STAT5.Compare the CD4+ T lymphocyte subsets and JAK2,STAT3,STAT5 expression level differences among two groups children and further analysis the correlation of JAK2-STAT3/ STAT5 signal transduction pathway and CD4+ T lymphocyte subsets.Results:The percentage of Th1 and Treg lymphocytes and Th1/ Th2 and Treg/ Th17 ratio of the group A,B,C in control group were significantly higher than the allergy group,while Th2,Th17 lower than allergy group.The expression mRNA level of JAK2,STAT3 and STAT5 in the control group were significantly lower than the allergic group,the difference was statistically significant(P<0.05).The correlation for JAK2,STAT3,STAT5 and CD4+ T level of lymphocyte subsets were compared,correlation coefficient of Th1,Treg,Th1/ Th2,Treg/ Th17 were all higher than 0(P<0.05),and the correlation coefficient of Th2 and Th17 were smaller than 0(P<0.05).However in group D,there was no significance.Conclusion:The JAK2-STAT3/ STAT5 signaling pathway in young children with food allergy has obvious activation and CD4+ T lymphocyte subsets change,and the JAK2-STAT3/ STAT5 signaling pathway can effectively regulate the distribution of CD4+ T lymphocyte subsets.With the age of children increasing,the correlation of these effects is weakened and disappeared.