白介素17 受体mRNA 在恒河猴中的分布 |
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引用本文: | 王凤杰,李冬,余磊,崔艳芳,叶华虎,法云智,杨贵波. 白介素17 受体mRNA 在恒河猴中的分布[J]. 中国免疫学杂志, 2018, 34(1): 19 |
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作者姓名: | 王凤杰 李冬 余磊 崔艳芳 叶华虎 法云智 杨贵波 |
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作者单位: | 中国疾病预防控制中心性病艾滋病预防控制中心;军事医学科学院实验动物中心; |
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摘 要: | 目的:阐述白介素17 受体(IL-17R)mRNA 在恒河猴不同组织中的分布与丰度。方法:建立实时荧光定量PCR 方法,检测免疫、消化和生殖等系统的各组织中IL-17RA mRNA 的水平,并检测了肠道IL鄄17RC mRNA 的水平及恒河猴、非洲绿猴和食蟹猴PBMC 中IL-17RA 和IL-17RC mRNA 的表达水平。结果:IL-17RA mRNA 在所有检测过的组织中均有表达,但不同器官系统的表达水平存在显著差异(P<0.05);IL-17RC mRNA 在大肠中的表达水平高于小肠;恒河猴PBMC 中IL-17RA mRNA 水平显著高于非洲绿猴(5 倍)和食蟹猴(3 倍),但PBMC 中IL-17RC mRNA 水平在3 种动物中均低于检测下限。结论:IL-17RA 广泛分布于恒河猴各种组织中,表达水平存在明显的组织差异和种属差异。
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关 键 词: | 白介素17 受体 实时荧光定量PCR 组织分布 恒河猴 非洲绿猴 |
Distribution of interleukin 17 receptor mRNA in rhesus macaques |
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Abstract: | Objective:To delineate the distribution and abundance of interleukin 17 receptor mRNA in normal tissues of rhesus macaques.Methods: Tissue samples were collected from different systems including lymphoid system,digestive system and genital system and the mRNA levels of IL-17RA and IL-17RC were examined by real time RT-PCR.Meanwhile the levels of IL-17RA and IL-17RC mRNA in the PBMCs of rhesus macaques,African green monkeys and cynomolgus monkeys were examined.Results: IL-17RA mRNA was expressed in all the tested tissues and the levels were significantly different among these systems (P<0.05).IL-17RC mRNA level in the large intestine was higher than that in the small intestine.IL-17RA mRNA level in rhesus macaque PBMCs was 3 and 5 times higher than that of cynomolgus monkeys and African green monkeys respectively,while IL-17RC mRNA in the PBMCs was below the limit of detection.Conclusion: IL-17RA is widely distributed in rhesus macaques;the expression level is different among different tissues and species. |
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