双歧杆菌联合乌司他丁对脓毒症模型大鼠免疫功能的影响

目的：观察双歧杆菌联合乌司他丁对脓毒症大鼠免疫功能的影响。 方法：将100只雄性成年SD大鼠随机分为假手术组、模型组、双歧杆菌治疗组、乌司他丁治疗组、联合治疗组。采用盲肠结扎穿孔术(CLP)制备脓毒症大鼠模型，观察记录各组大鼠的生存数量，测定肠道菌群的数量，采用酶联免疫吸附法(ELISA)检验肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)，采用流式细胞术检测T细胞亚群，测定血浆内毒素。结果：模型组大鼠血浆内毒素、肠道大肠杆菌、CD8⁺T 细胞、TNF-α和IL-1β的含量显著高于假手术组（P<0.05），CD3⁺、CD4⁺T细胞、CD4⁺/CD8⁺T细胞比值、双歧杆菌和乳酸杆菌显著低于假手术组(P<0.05)；与模型组比较，联合治疗组的肠道血浆内毒素、大肠杆菌、CD8+T细胞、TNF-α和IL-1β的含量显著下降(P<0.05)，CD3⁺、CD4⁺T细胞、CD4⁺/CD8⁺T细胞比值、双歧杆菌和乳酸杆菌含量显著升高(P<0.05)。联合治疗组中肠道血浆内毒素、TNF-α和IL-1β的含量显著低于双歧杆菌治疗组和乌司他丁治疗组(P<0.05)，CD3⁺、CD4⁺、CD4⁺/CD8⁺T细胞比值、乳酸杆菌含量显著优于双歧杆菌治疗组和乌司他丁治疗组。结论：双歧杆菌和乌司他丁联合使用疗效显著提高，抑制CLP大鼠的TNF-α、IL-6的表达，降低血浆内毒素的水平，调节肠道菌群平衡，可提高脓毒症模型大鼠的免疫功能。

Effect of Bifidobacterium combined with ulinastatin on immunologic function of CLP rats

Objective:To investigate the effect of Bifidobacterium combined with ulinastatin on the immunologic function of sepsis rats. Methods: One hundred male SD rats were randomly divided into sham-operated group,model group,Bifidobacterium treatment group,Ulinastatin treatment group,combining treatment group.Cecal ligation and punctured was used to prepare sepsis model in rats.Survival rate was observed.The number of intestinal floras were determined.The tumour necrosis factor-α (TNF-α)and interleukin-6 (IL-6)were tested by enzyme linked immunosorbent assay (ELISA).T-lymphocyte subset was tested by flow cytometry.Serum endotoxin was tested by TAL method.Results: Serum endotoxin,Escherichia coli,CD8⁺T cell,TNF-α and IL-1β of model group were significantly higher than the sham operation group (P<0.05).CD3⁺，CD4⁺T cell and ratio of CD4⁺/CD8⁺T cell,Bifidobacterium and lactobacillus were significantly higher than the sham operation group (P<0.05).Compared with model group,Serum endotoxin,Escherichia coli,CD8⁺T cell,TNF-α and IL-1β of combining treatment group were significantly decrease (P<0.05).CD3⁺，CD4⁺T cell and ratio of CD4⁺/CD8⁺T cell,Bifidobacterium and lactobacillus were significantly increase (P<0.05).Serum endotoxin,TNF-α and IL-1β of combining treatment group were significantly lower than that in Bifidobacterium treatment group and Ulinastatin treatment group.CD3⁺，CD4⁺T cell and ratio of CD4⁺/CD8⁺T cell,Bifidobacterium and lactobacillus were significantly higher than that in Bifidobacterium treatment group and Ulinastatin treatment group. Conclusion: Efficacy is significantly improvement while Bifidobacterium combined with ulinastatin,which can restrain TNF-α and IL-6 of CLP rats,decrease the increasing of serum endotoxin,and regulate the balance of intestinal floras,also improve the immune function of CLP rats.