XKRX在结直肠癌的表达和临床意义

目的　探讨XKRX（XK related, X-linked）在结直肠癌组织中的表达和临床意义。 方法　收集TCGA公共数据库中结直肠癌芯片数据，对结直肠癌组织样本中XKRX基因表达数据以及对应的临床资料进行回顾性分析和生存分析。在临床上收取结直肠癌样本，通过实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹（western blot）实验验证XKRX表达水平是否与芯片结果一致，为研究XKRX基因提供新的实验数据。 结果　芯片结果显示XKRX在结直肠癌组织的表达水平明显高于癌旁正常组织（P＜0.0001）。利用实时荧光定量PCR和蛋白免疫印迹实验检测21例结直肠癌临床样本中XKRX表达水平，结果显示结直肠癌组织中XKRX的信使RNA（mRNA）和蛋白质表达水平明显高于癌旁正常组织。XKRX表达与性别（P=0.9034）、年龄(P=0.886)、TMN分期(P=0.3979)、淋巴结转移(P=0.7995)和远处转移(P=0.6032)无明显统计学差异。但XKRX高表达组的生存时间明显低于低表达组（P=0.0075）。 结论　XKRX在结直肠癌组织中表达上调，可能发挥原癌基因的作用，影响结直肠癌的恶性进展，进而降低患者的生存时间，提示患者不良预后。

Expression and clinical significance of XKRX in colorectal cancer

Objective　To explore the expression and clinical significance of XK related, X-linked (XKRX) in colorectal cancer tissue.　Methods　The gene expression of XKRX and corresponding clinical data in colorectal cancer in TCGA public database were collected to make retrospective analysis and survival analysis. Quantitative real-time PCR and western blot were conducted to assess the expression of XKRX in 21 cases of colorectal cancer tissues.　Results　We found that the expression level of XKRX in colorectal cancer tissues was significantly higher than that of adjacent tissues (P<0.0001). The mRNA and protein expression level of XKRX in colorectal cancer tissues were significantly higher than that of the tissues adjacent to carcinoma. There was no significant difference between XKRX expression and gender (P= 0.9034), age (P=0.886), TMN stage (P=0.3979), lymph node metastasis (P=0.7995) and distant metastasis (P=0.6032). However, the survival time of the group with XKRX high expression was significantly lower than that of the group with low expression (P=0.0075).　Conclusion　Our data shows that XKRX is up-regulated in colorectal cancer tissues and may play a role in the proto-oncogene, which may affect the malignant progression of colorectal cancer and reduce the survival time of patients.