下调HIF-2α基因对低氧诱导的肝癌细胞体外生物学行为的影响 |
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引用本文: | 李伟伟,耿晓松,申保生,杨玉新,宋新文.下调HIF-2α基因对低氧诱导的肝癌细胞体外生物学行为的影响[J].中国免疫学杂志,2018,34(2):199. |
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作者姓名: | 李伟伟 耿晓松 申保生 杨玉新 宋新文 |
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作者单位: | 新乡医学院第一附属医院感染疾病科;新乡医学院护理学院; |
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摘 要: | 目的:探讨下调缺氧诱导因子2α(Hypoxia-inducible factor 2α,HIF-2α)基因对人肝癌细胞株HepG2 低氧状态下增殖、凋亡、细胞周期分布和迁移侵袭力的影响。方法:选取氯化钴(CoCl2 )诱导的人肝癌细胞株HepG2 作为研究对象,构建HIF-2αsiRNA 特异性载体,转染低氧环境下的HepG2 细胞,Real-time PCR 和Western blot 方法分别检测转染前后细胞中HIF-2αmRNA 和蛋白表达,MTT 方法检测转染前后HepG2 细胞增殖,流式细胞术检测转染前后HepG2 细胞凋亡和细胞周期分布,Transwell 试验检测转染前后HepG2 细胞侵袭迁移能力。结果:低氧诱导下,肝癌HepG2 细胞HIF-2α表达显著增多;特异性转染HIF-2αsiRNA 于低氧环境下的HepG2 细胞后,HIF-2αmRNA 和蛋白表达水平均受到明显抑制、细胞增殖能力降低,凋亡率升高,分布于G0/ G1 期细胞比率升高,合成期(S)及合成后期(G2/ M)细胞比率降低,细胞体外侵袭迁移能力受到明显抑制(P<0.05)。结论:低氧环境下肝癌HepG2 细胞表达HIF-2α增多,特异性siRNA 可通过下调低氧环境下HepG2 细胞中HIF-2α基因表达,抑制HepG2 细胞增殖、侵袭迁移,改变细胞周期分布并诱导其凋亡。
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关 键 词: | 肝癌 缺氧诱导因子2α RNA 干扰 侵袭 细胞凋亡 |
Effects of downregulation of HIF-2α gene on biological behaviors of hepatoma cells induced by hypoxia in vitro |
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Abstract: | Objective:To explore the effects of hypoxia-inducible factor 2αgenes on under hypoxia on proliferation,apoptosis, cell cycle distribution and migration of invasiveness of human hepatocellular carcinoma cell HepG2.Methods: Human hepatoma cell line HepG2 induced by cobalt chloride (CoCl2 ) was selected as the research object,construction of siRNA specific carrier HIF-2α,transfection of HepG2 cells under hypoxia.Real-time PCR,Western blot method in the detection of before and after transfection in each group of HIF-2αmRNA and protein expression;MTT method to detect the proliferation of HepG2 cells before and after transfection;apoptosis rate and distribution of cell cycle of HepG2 cells before and after transfection were detected by flow cytometry;Transwell test was used to detect the invasion and migration ability of HepG2 cells before and after transfection.Results: Under hypoxia,significant increased HIF-2αexpression in hepatocellular carcinoma HepG2 cells.Specific transfection of HIF-2αsiRNA in HepG2 cells after HIF-2αmRNA and protein expression levels were significantly inhibit cell proliferation decreased,apoptosis rate increased in the ratio of G0/ G1 phase cells increased synthesis phase (S) and late (G2/ M) synthesis cell ratio decreased,which in vitro invasion and migration of cells was inhibited.Conclusion: Expression of HIF-2αincreases in hepatocellular carcinoma HepG2 cells under hypoxia. Specific siRNA can be cut by HIF-2αgene expression in HepG2 cells under hypoxia,to inhibit HepG2 cell proliferation,invasion,migration,and change the distribution of cell cycle and induce its apoptosis. |
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