旋毛虫对不同肠炎模型小鼠脾脏淋巴细胞Th1/Th2水平的影响

目的：研究旋毛虫（T.spiralis）对肠炎小鼠［三硝基苯磺酸（TNBS）或唑酮（OXZ）诱导］脾脏淋巴细胞中Th1/Th2水平的影响。方法：雌性BALB/c小鼠，随机分为50%乙醇对照组、TNBS(OXZ)诱导肠炎模型组、预先感染T.spiralis后诱导TNBS(OXZ)模型组，每组小鼠取材时保证6只以上。对各组小鼠脾脏淋巴细胞进行分离，采用流式细胞术观察TNBS（OXZ）诱导肠炎模型组和预先感染T.spiralis后诱导TNBS（OXZ）模型组。造模后3 d和7 d脾脏淋巴细胞中Th1/Th2水平的变化。结果：与模型组相比，预先感染T.spiralis后诱导TNBS肠炎第3天脾脏Th1/Th2比值未见明显下降（P>0.05），于第7天明显降低（P<0.05）。诱导OXZ肠炎模型后第3天及第7天小鼠脾脏Th1/Th2比值均明显低于T.spiralis干预组（P<0.05）。结论：针对TNBS 肠炎模型，T.spiralis可能是通过诱导Th2及Tr1型免疫反应抑制模型小鼠过度的Th1型免疫而起到良好的干预作用。在T.spiralis对OXZ模型小鼠的干预性研究中，并无T.spiralis感染诱发的Th2型炎症反应加重同样以Th2升高为主的OXZ模型小鼠的病情。

Levels of Th1/Th2 in spleen lymphocyte of colitic mice without or with Trichinella spiralis infection

Objective:To study the levels of Th1 and Th2 in spleen lymphocyte of TNBS and OXZ colitis without or with Trichinella spiralis(T.spiralis)infection. Methods: Female BALB/c mice were randomly divided into 3 groups:50% Ethanol,TNBS or OXZ,T.spiralis+TNBS or OXZ(at least 6 in each group when mice were killed).The ratio of Th1/Th2 lymphocyte in spleen was detected by FCM with three color intracellular cytokine staining. Results: In spleen lymphocyte of TNBS colitic mice with prior nematode infection,the ratio of Th1/Th2 were not significantly different compared with that seen in colitic mice without prior nematode infection on day 3 post-induction of colitis（P>0.05）,the ratio of Th1/Th2 was significantly lower than which on day 7 post-induction of colitis（P<0.05）.In spleen lymphocyte of OXZ colitic mice with prior nematode infection on days 3 and 7 post-induction of colitis,the ratio of Th1/Th2 was significantly higher than that seen in colitic mice without prior nematode infection（P<0.05）.Conclusion: Infection of mice with T.spiralis distracts the mucosal immune system response from a Th1 response toward a protective Th2 response and up-regulate Tr1-cytokines with an attendant reduction in the severity of colonic damage and balance of Th1/Th2.Prior T.spiralis infection doesn′t reduce the severity of OXZ-induced colitis,but without aggravating colitis.