乌头碱对肝癌MHCC97 细胞生长、侵袭和迁移的调控作用及机制研究

目的:探究乌头碱对肝癌细胞生长、侵袭和迁移的作用机制。方法:MTT 法检测不同浓度乌头碱对人肝癌细胞(MHCC97)增殖的影响;Transwell 检测乌头碱对MHCC97 细胞侵袭能力的影响;划痕实验检测乌头碱对MHCC97 细胞迁移能力的影响;Western blot 检测乌头碱对P38MAPK 通路蛋白表达的影响。结果:根据预实验结果,选择5、10、20 μg/ ml 三个浓度进行后续实验。乌头碱(10、20 μg/ ml)作用4 d 后对MHCC96 细胞增殖有明显的抑制作用,并能明显降低肝癌细胞的侵袭能力。乌头碱作用于MHCC97 细胞后,细胞迁移能力明显减弱,p-P38/ P38 比值降低,p-MAPKAPK 和p-HSP27 表达明显减少。结论:乌头碱对肝癌细胞增殖、侵袭和迁移具有抑制作用,其作用机制可能与调控P38/ MAPK 信号通路激活有关。

Regulatory effects and mechanism of aconitine on proliferation,invasion and migration of hepatoma carcinoma cell MHCC97

Objective:To investigate the anticancer activity and mechanism of aconitine on cell proliferation,invasion and migration of hepatoma carcinoma cell(HCC).Methods:The effect of aconitine at different concentrations on proliferation was calculated by MTT assay.The effects of aconitine on invasion and migration of HCC were measured by Transwell and wound healing assay.Western blot was employed to detect the protein levels of P38MAPK signaling pathway-related proteins.Results:The concentrations of 5,10,20μg/ ml were selected according to the results of pre-experiment.Aconitine (10,20 μg/ ml) inhibits proliferation and invasion of MHCC97 cells markedly after cells were treated with aconitine for 4 days.Treatment with aconitine down-regulated the ability of migration and decreased the ratio of p-P38/ P38 and protein levels of p-MAPKAPK and p-HSP27.Conclusion:Aconitine inhibits proliferation,invasion and migration,and the mechanism may related with P38MAPK signaling pathway.