枸杞多糖对免疫抑制小鼠红细胞免疫功能的影响

目的:探讨枸杞多糖对阿霉素化疗小鼠红细胞免疫功能的影响。方法:取BALB/ c 小鼠给予阿霉素化疗以制备免疫抑制模型,并灌服枸杞多糖(62.5、125、250 mg/ kg)干预处理7 d,实验同时设置正常对照组和模型组。采用流式细胞术检测红细胞膜CD59 表达水平;采用考马斯亮蓝染色法观察红细胞膜Band鄄3 蛋白表达;采用Western blot 法检测红细胞NKEF-A、NKEF-B 表达水平;流式细胞术检测脾NK 细胞杀伤H22 细胞的能力。结果:阿霉素化疗后小鼠红细胞Band-3 蛋白、NKEF-A、NKEF-B 表达水平和NK 细胞杀伤活力均显著降低,而CD59 分子表达未见显著变化。枸杞多糖对化疗小鼠红细胞Band-3蛋白表达、NKEF-A 和NKEF-B 表达水平均有显著恢复作用,并能恢复化疗小鼠NK 细胞杀伤活性。结论:枸杞多糖可以改善化疗小鼠红细胞免疫功能。

Effect of Lycium Barbarum polysaccharides on immune function of erythrocytes in immunosuppressed mice

Objective:To investigate the effect of Lycium Barbarum polysaccharides on immune function of erythrocytes in doxorubicin-treated mice.Methods: BALB/ c mice were treated with doxorubicin and used as immunosuppression model.The mice were treated with Lycium Barbarum polysaccharides(62.5,125,250 mg/ kg) for 7 days,the normal control mice and model control mice were also used in this study.Expression level of CD59 molecule in erythrocytes was analyzed with flow cytometry.The Band-3 level was analyzed by Coomassie Brilliant Blue method.The NKEF-A and NKEF-B expression level in erythrocytes was analyzed by Western blot.The killing activity of NK cells was analyzed with flow cytometry.Results: The level of Band-3,NKEF-A and NKEF-B was decrease in erythrocytes of doxorubicin-treated mice.The killing activity of NK cells was also decrease in the mice when the expression level of CD59 molecule was not change obviously.Lycium Barbarum polysaccharides treatment could promote the recovery of Band-3, NKEF-A and NKEF-B in erythrocytes of the mice.Conclusion: Lycium Barbarum polysaccharides can promote the recovery of immune function of erythrocytes in doxorubicin-treated mice.