肿瘤抗原特异性 TCR 基因筛选

抗原特异性T 细胞受体修饰T 细胞(TCR-T)的过继性疗法已成为肿瘤生物治疗的研究热点,成功获得肿瘤抗原特异性的TCR 基因是肿瘤TCR-T 细胞治疗的重要前提。本文将聚焦于基于多细胞RT-PCR 扩增、基于单细胞RT-PCR 扩增和基于TCR 基因CDR3 片段长度多态性分析等几个方面简要介绍肿瘤特异性TCR 基因的筛选。

A brief introduction of identification of tumor antigen-specific TCR gene

Adoptive therapy with T cells modified by tumor antigen specific T cell receptor(TCR)gene become a research hotspot in tumor biotherapy.The generation of high-affinity TCR is a significant pre-condition for TCR-T therapy.In this review,we focus on the identification of tumor antigen-specific TCR genes,which based on RT-PCR,single cell RT-PCR and CDR3 length polymorphism analysis.