还原型谷胱甘肽对活性氧介导的系统性红斑狼疮患者中性粒细胞NETosis的影响

目的　探讨抗氧化剂还原型谷胱甘肽（GSH）能否通过调控中性粒细胞ROS水平从而抑制中性粒细胞发生NETosis，减少中性粒细胞胞外诱捕网（neutrophil extracellular traps, NETs）的形成，为临床开发新的治疗系统性红斑狼疮（SLE）的药物提供理论基础。 方法　采用梯度离心法分离SLE患者外周血中性粒细胞，利用佛波酯（PMA）诱导中性粒细胞发生NETosis后，检测各组中性粒细胞胞内ROS水平并观察其NETs产生情况。 结果　与PMA处理组比较，经过GSH预处理的SLE患者中性粒细胞体外诱导NETs产生明显减少。同时，GSH也能明显降低SLE患者中性粒细胞胞内ROS水平。 结论　GSH可能通过调控SLE患者中性粒细胞ROS的水平，从而抑制其发生NETosis，提示GSH可能在SLE治疗中发挥作用。

The effect of reduced glutathione on the ROS-mediated NETosis by neutrophils from patients with systemic lupus erythematosus

Objective　Evidence has demonstrated that the formation of neutrophil extracellular traps (NETs) formation mediated by neutrophil plays an important role in the pathogenesis and progression of systemic lupus erythematosus (SLE). In this study, we aimed to investigate whether reduced glutathione (GSH) could decrease the level of intracellular reactive oxygen species (ROS) or inhibit NETs formation. Methods　Neutrophils were isolated from peripheral blood of SLE patients by gradient centrifugation method dextran sedimentation and centrifugation, and then were stimulated with phorbol 12-myristate 13-acetate (PMA). The production of intracellular ROS and the formation of NETs were examined. Results Compared with the PMA group treated with PMA, the group preprocessing with GSH could markedly suppress formation of NET by neutrophils from SLE patients. Moreover, the production of intracellular ROS production was significantly decreased in GSH-treated neutrophils from SLE patients. Conclusion　GSH can significantly inhibit the process of neutrophils' NETosis from SLE patients through modulating the intracellular ROS production. These results indicates that GSH might be a potential therapeutic agent for SLE.