姜黄素抑制CD44+结肠癌细胞增殖的体外实验研究

目的:探讨姜黄素能否抑制CD44+ 结肠癌细胞的增殖。方法:MTT 法检测姜黄素处理的结肠癌细胞增殖情 况,Real-time PCR 检测姜黄素处理的结肠癌细胞CD44 mRNA 的表达水平,流式细胞术检测姜黄素处理的结肠癌细胞表面 CD44 的表达水平,磁珠分选方法分离得到结肠癌细胞中CD44+ 细胞和CD44- 细胞, MTT 法观察姜黄素对两种细胞增殖情况 的影响。结果:姜黄素可抑制结肠癌细胞HCT116 和HT鄄29 增殖,Real鄄time PCR 和流式细胞术均证明姜黄素可导致结肠癌细 胞CD44 表达下调,磁珠分选方法获得CD44+的结肠癌细胞并应用姜黄素处理后,结果提示姜黄素可抑制CD44+细胞增殖。结 论:姜黄素可通过抑制结肠癌肿瘤干细胞增殖而降低结肠癌复发和转移,有望成为结肠癌治疗的辅助性药物。

Proliferation inhibition effects of curcumin against CD44+ colon cancer cells#br# in vitro

Objective:To study confirm whether curcumin could inhibit the proliferation of CD44+ colon cancer cells.In this study.Methods: MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells.And Real-time PCR and Fluorescence-activated cell sorting (FACS) were used to determine CD44 expression level in colon cancer cells after treated with curcumin.Then we used Magnetic-activated cell sorting (MACS) method to separate CD44 positive and negative cells.And we compared the proliferation inhibition effects of curcumin against these two kinds of cells.Results: Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines.The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin.Conclusion: Curcumin could inhibit the proliferation of CD44+ colon cancer cells.Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.