CRISPR / Cas9 基因编辑技术在肿瘤免疫治疗中的应用

近年来肿瘤免疫治疗因其显著疗效和创新性备受关注,是肿瘤治疗中最具有前景的研究方向之一。CRISPR基因编辑系统的发展以及其在多领域的延伸应用让研究者看到了它的巨大潜力,在肿瘤免疫治疗研究中也显示出广泛的应用前景。在过继性细胞治疗中,CRISPR/ Cas9 可对TCR-T 和CAR-T 细胞的内源性TCR 以及HLA-Ⅰ分子的基因进行敲除得到通用型效应细胞;在基于免疫检查点阻断的治疗中,其为免疫检查点的抑制和阻断提供了一种新的方法;同时在抗体靶向疗法中,CRISPR/ Cas9 技术在候选靶点筛选及简化抗体制备流程中有着重要应用。CRISPR 从各方面极大地推动了肿瘤免疫治疗的研究,本文将对CRISPR/ Cas9 基因编辑系统在肿瘤免疫治疗方面的应用进行介绍。

Application of CRISPR / Cas9 in tumor immunotherapy

In recent years,tumor immunotherapy has attracted more and more attention because of its remarkable curative effect and innovation.With the development of CRISPR gene editing system and its application in many fields,researchers have seen its great potential,especially in the field of cancer research.For adoptive cell therapy,CRISPR/ Cas9 can knockout endogenous TCR and HLA-I genes in TCR-T and CAR-T cells to generate universal effective T cells.It provided a new method to inhibit and block the immune checkpoint.The application of CRISPR/ Cas9 in genetic screening makes antibody targeted therapy to a new level.CRISPR has greatly promoted the research of tumor immunotherapy.This article introduced the application of CRISPR/ Cas9 gene editing system in tumor immunotherapy.