miR-217调控lncRNAMALAT1影响食管鳞癌细胞的生物学行为

目的　探讨miR-217通过调控lncRNA MALAT1抑制食管鳞状细胞癌细胞的增殖，迁移和侵袭行为及其机制。 方法　qPCR检测miR-217在食管鳞状细胞癌组织和不同细胞株中的表达情况；双荧光素酶报告基因检测miR-217与MALAT1之间的相互作用；MTT增殖实验检测抑制miR-217后食管鳞状细胞癌细胞的增殖能力的变化情况；划痕愈合试验和Transwell侵袭实验检测抑制miR-217后食管鳞状细胞癌细胞的迁移和侵袭行为的变化情况；Western blotting实验检测miR-217对MALAT1下游相关蛋白表达情况的影响。 结果　与正常食管组织相比，食管鳞状细胞癌组织中miR-217的表达水平相对上调，与其他细胞株相比，Ec109细胞中miR-217表达最高；双荧光素酶实验证实miR-217能与MALAT1的3’ UTR特异性结合，可以调控MALAT1的表达与活性；抑制miR-217的表达后可以抑制食管鳞状细胞癌细胞的增殖、迁移和侵袭能力；抑制miR-217后MALAT1下游MIA2，ROBO1表达明显下调。 结论　miR-217可以调控MALAT1的表达影响食管鳞状细胞癌细胞的生物学行为。

miR-217 inhibits proliferation,migration, and invasion of esophageal squamous cell carcinoma cells by regulating lncRNA MALAT1

Objective　To investigate the inhibitory effect of miR-217 on proliferation, migration and invasion of esophageal squamous cell carcinoma cells by regulating lncRNA MALAT1.　Methods　qPCR was used to detect the expression of miR-217 in esophageal squamous cell carcinoma and different cell lines. Dual luciferase reporter assay detected the interaction between miR-217 and MALAT1. MTT assay was used to detect the proliferation of esophageal squamous cell carcinoma cells after miR-217 was inhibited. Scratch healing assay and Transwell invasion assay were used to detect the migration and invasion of esophageal squamous carcinoma cells after miR-217 was inhibited. The effect of miR-217 on the expression of MALAT1 downstream protein was detected by Western blotting.　Results　Compared with normal esophageal tissue, the expression of miR-217 in esophageal squamous cell carcinoma was relatively up-regulated, and the expression of miR-217 was the highest in Ec109 cells compared with other cell lines. Dual luciferase assay confirmed that miR-217 could specifically bind to 3'UTR of MALAT1 and regulate the expression and activity of MALAT1. Inhibition of miR-217 expression could inhibit esophageal squamous cell carcinoma cell proliferation, migration and invasion ability. Down-regulation of miR-217 after MALAT1 MIA2, ROBO1 expression was significantly down-regulated.　Conclusion　miR-217 can regulate the expression of MALAT1 and affect the biological behavior of esophageal squamous cell carcinoma cells.