依达拉奉联合灯盏花素对脑缺血大鼠大脑皮质MCP-1的影响

目的　观察大鼠脑缺血（MCAO）后大脑皮质MCP-1的表达变化以及用依达拉奉联合灯盏花素干预后对MCP-1表达的影响。 方法　复制大鼠大脑中动脉闭塞（MCAO）模型，应用RT-PCR、Western blot和免疫荧光技术检测大鼠MCAO及药物干预后MCP-1的表达变化。 结果　RT-PCR显示大鼠MCAO后大脑皮质MCP-1 mRNA的表达比对照组显著上升（P<0.05），12 h达高峰，与1 d、3 d及1周组比有显著差异（P<0.05）；给予两种药物联合处理后，mRNA表达显著降低，与对照组及单独用药组相比均有显著差异（P<0.05）。 Western blot显示MCAO 1 d、3 d、1周大脑皮质MCP-1蛋白的表达显著增强，与对照组比有显著差异（P<0.05）；两种药物联合处理后，MCAO大鼠大脑皮质MCP-1蛋白表达明显减少，与对照组及单独用药相比均有显著差异（P<0.05）。免疫荧光染色显示MCAO大鼠缺血半暗带有大量激活小胶质细胞，一部分与MCP-1免疫阳性细胞有共表达，其中MCAO后1周组最明显。 结论　大鼠局灶性脑缺血（MCAO）后，依达拉奉联合灯盏花素治疗能有效减少大脑皮质MCP-1的表达，效果优于两种药物单独使用。

The effects of edaravone combined with breviscapine treatment on MCP-1 expression in the cerebral cortex after focal cerebral ischemia in rats

Objective　This research investigated the expression of MCP-1 in cerebral cortex of rat following middle cerebral artery occlusion (MCAO) and edaravone combination with breviscapine as a treatment of MCP-1 expression.　Methods　Animal models of MCAO were established， and RT-PCR，Western blot and Immunohistochemistry were used to detect the expression of MCP-1 in cerebral cortex of rats after MCAO and drug intervention. Results The expression of MCP-1 mRNA were significantly enhanced after MCAO, and became most severe at 12 h, and the difference was significant (P<0.05). The expression of MCP-1 mRNA was markedly decreased compared with the saline group after drug treatment (P<0.05)； The combination of edaravone plus breviscapine was more effective than edaravone or breviscapine treatment alone (P<0.05). The expression of MCP-1 protein was significantly increased in 1d, 3d, 1 week following MCAO and stayed at a high level (P<0.05), and than obviously decreased compared with the saline group after drug treatment (P<0.05); the combination of edaravone plus breviscapine was more effective than edaravone or breviscapine alone (P<0.05) . Immunofluorescence staining showed that activated microglial cells was co-labeled with MCP-1 positive cells, and the number of MCP-1 positive cells was significantly increased after MCAO peaking at 1week. By the mean time, microglial cells did not exhibit MCP-1 positive cells in saline group.　Conclusion　Edaravone combination with breviscapine as a treatment could decrease the MCP-1 expression after cerebral ischemia, and the combination of edaravone plus breviscapine is more effective than edaravone or breviscapine alone.