额叶p-mTOR异常表达与外伤性癫痫发生的相关研究

目的:探索外伤性癫痫(Posttraumatic epilepsy,PTE)发生与额叶p-mTOR异常表达的相关性。方法:将78只成年雄性SD大鼠分为正常对照组(A组)6只,生理盐水对照组(B组)和癫痫模型组(C组)各36只,C组右侧额叶注射FeCl_2造模。应用免疫组化技术检测造模后1h、24h、1周、2周、4周各组大鼠额叶皮质中p-mTOR(Ser2448)的动态表达。结果:A组额叶p-mTOR(Ser2448)表达的免疫组织化学染色,神经元胞质呈浅棕色,提示弱阳性表达;B组细胞染色程度较A组加深,p-mTOR(Ser2448)表达阳性细胞数增加;C组可见细胞结构紊乱,胞质呈明显的棕黄色,提示该蛋白在细胞质中呈强阳性表达。C组和B组比较,1h表达开始升高(t=-1.435,P=0.182),但差异无统计学意义,1周表达高峰(t=-4.073,P=0.002),2周降低(t=-2.614,P=0.026),4周时再次升高(t=-2.506,P=0.031),差异具有统计学意义(均P0.05)。结论:PTE的发病机制可能与mTOR信号通路上pmTOR(Ser2448)的表达异常增加有关。

Assessment of the correlation between posttraumatic epilepsy and the abnormal expression of p-mTOR in frontal lobe

Objective: To explore the correlation between the abnormal expression of p-mTOR in the frontal lobe and posttraumatic epilepsy (PTE). Methods: Adult male SD rats were randomly divided into control group (n=6), saline group (n=36) and epilepsy group (n=36). Models were established by injecting FeCl2 into the right frontal lobe. Immunohistochemistry was used to detect the dynamic expression of p-mTOR (Ser2448) in the frontal cortex of rats at different time points (1 h, 24 h, 1 week, 2 weeks, and 4 weeks after modeling). Results: As compared with the saline group, the expression of p-mTOR (Ser2448) in the frontal cortex of the epilepsy group began to increase after 1 h (t=-1.435, P=0.182), reached its maximum after 1 week (t=-4.073, P=0.002), reduced after 2 weeks (t=-2.614, P=0.026) and rose again after 4 weeks (t=-2.506, P=0.031). The difference was statistically significant. Conclusion: The pathogenesis of PTE may be related to the abnormal increase of the expression of p-mTOR (Ser2448) protein in the mTOR signaling pathway.