| HPLC测定消渴灵中盐酸小檗碱的含量 |
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| 引用本文： | 黄新兴,赵秀艳.HPLC测定消渴灵中盐酸小檗碱的含量[J].中国中医药现代远程教育,2011,9(5):186-187. |
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| 作者姓名： | 黄新兴 赵秀艳 |
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| 作者单位： | 吉林省通化市食品药品检验所仪器室,通化,134000 |
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| 摘 要： | 目的建立高效液相色谱法测定消渴灵片中盐酸小檗碱的含量。方法采用反相高效液相色谱法,选用Dimensions C18(250mm×4.6mm;5μm)色谱柱,流动相为0.05mol/L磷酸二氢钾-乙腈(64:36),流速为0.8mL·min-1,柱温25℃,检测波长为265nm。结果盐酸小檗碱在0.039～0.394μg·mL-1范围内呈良好线性,r=0.9999,方法平均回收率为96.14%,RSD=0.2%。结论方法简便,快速,结果准确,分离效果好。
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| 关 键 词： | 消渴灵片 盐酸小檗碱 HPLC 含量测定 |
Determination of Berberine Hydrochloride In Xiaokeling Tablets by HPLC |
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| Huang XinXing,Zhao Xiuyan.Determination of Berberine Hydrochloride In Xiaokeling Tablets by HPLC[J].Chinese Medicine Modern Distance Education of China,2011,9(5):186-187. |
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| Authors: | Huang XinXing Zhao Xiuyan |
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| Institution: | Huang XinXing,Zhao Xiuyan(Jlin Tonghua Institute for Drug Control,Tonghua 134000) |
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| Abstract: | Objective To develop an HPLC mehod for determination of Berberine Hydrochloride in Xiaokelingo Tablets.Methods The analytical Column was a Dimensions C18（250m×4.6 mm,5μm）The mobile phase consisted of 0.05mol/Lmonopotassium phosphate-Acetonitrile（64：36）,The flow rate was 0.8mL min-1 and the detection wavelength was 265nm.The column temperature was 25 ℃.Results Imperatorin presents a good Line arity（r=0.999 9） within the range of 0.039～0.394μg.mL-1 The average kecovery rate were 96.14% and the RSD = 0.2%.Conlusions The method is simple,fast,accurate and with a good separating results. |
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| Keywords: | HPLC |
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