| 时间分辨荧光免疫法定量检测AFP试剂盒临床应用研究 |
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| 引用本文： | 徐伟文,吴英松,杭建峰,王从容,周志聪,余伟鸿,裘宇容,李明.时间分辨荧光免疫法定量检测AFP试剂盒临床应用研究[J].标记免疫分析与临床,2006,13(1):43-45. |
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| 作者姓名： | 徐伟文 吴英松 杭建峰 王从容 周志聪 余伟鸿 裘宇容 李明 |
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| 作者单位： | 南方医科大学热带病研究室,广东,广州,510515;南方医科大学附属南方医院检验科,广东,广州,510515;广州市达瑞抗体工程技术有限公司,广东,广州,510663 |
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| 基金项目： | 广东省广州市创新条件建设项目 |
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| 摘 要： | 对自制AFP时间分辨荧光免疫法(TRFIA)试剂盒进行研究,为临床应用评价提供科学依据。收集血清标本450份,分别以化学发光免疫分析(CLIA)和RIA为对照,用AutoDELFIATMhAFP试剂盒为复核试验,测定血样,获取实验数据并计算“真实性”和“可靠性”。结果表明,CLIA及RIA为对照试验,其阳性符合率分别为99.0%和97.4%,阴性符合率分别为97.2%和94.1%,检验均无显著性差异(P>0.05)。本试剂盒的定量测定值与CLIA比较接近,相关系数为0.994,与RIA为0.917,两者相关性好(P<0.01)。本试剂盒检测性能能满足临床应用的需要。
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| 关 键 词： | 甲胎蛋白 时间分辨荧光免疫法 化学发光免疫分析法 放射免疫分析法 |
| 文章编号： | 1006-1703(2006)01-0043-03 |
| 收稿时间： | 2005-06-06 |
| 修稿时间： | 2005-09-22 |
The Clinical Application of the AFP Quantitative Diagnostic Kit Using TRFIA |
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| XU Wei-wen, WU Ying-song, HANG Jian-feng,et al..The Clinical Application of the AFP Quantitative Diagnostic Kit Using TRFIA[J].Labeled Immunoassays and Clinical Medicine,2006,13(1):43-45. |
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| Authors: | XU Wei-wen WU Ying-song HANG Jian-feng |
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| Institution: | The Dep. of Tropical Medicine, Nanfang Medical University, Guangzhou 510515,China |
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| Abstract: | AFP quantitative diagnostic kit was developed by TRFIA,and the capability of the kit was evaluated.In this study,450 blood samples were collected for AFP detection.Compared with the techniques of CLIA and RIA,the positive accordant ratios of our kit were 99.0% and 97.4%,and the negative accordant ratios were 97.2% and 94.1%,respectively.The accordance of our kit with both CLIA and RIA were good(P>0.05),and the accordance coefficients were 0.994 and 0.917,respectively.All the results of the parameters showed that the diagnostic kit we developed could meet the need of the clinic application. |
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| Keywords: | Alpha fetoprotein Timed-resolved fluoroimmunoassay Chemiluminescence immunoassay Radioimmunoassay |
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