| 用生物素标记Y特异DNA探针对一例Y染色体结构异常患者的研究 |
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| 引用本文： | 肖福英,刘希贤,杨真荣.用生物素标记Y特异DNA探针对一例Y染色体结构异常患者的研究[J].右江民族医学院学报,1992(3). |
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| 作者姓名： | 肖福英 刘希贤 杨真荣 |
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| 作者单位： | 右江民族医学院生物学教研室，同济医科大学医学遗传学研究室，同济医科大学医学遗传学研究室 |
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| 摘 要： | 用生物素标记Y特异3.4kbDNA为探针,应用染色体原位杂交技术对一例表型异常、常规染色体显带技术为小Y染色体的患者进行了研充:该患者不能与3.4kbDNA杂交,与~3H标记探针的染色体原位杂交结果一致。进一步以生物素标记此探针对患者的基因组DNA进行Southern印迹杂交分析,患者缺少一部分可检测的男性特异性片段,结合其临床特征,提示该患者为Yq的中间缺失。
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| 关 键 词： | 生物素 Y特异DNA探针 Y染色体 染色体原位杂交 Southern印迹杂交 |
Studies on a case of structured anomalies of Y chromosome by using biotinglated Y specific DNA probe |
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| Xiao Fuying Liu Xixian Yang Zhenrong.Studies on a case of structured anomalies of Y chromosome by using biotinglated Y specific DNA probe[J].Journal of Youjiang Medical College For Nationalities,1992(3). |
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| Authors: | Xiao Fuying Liu Xixian Yang Zhenrong |
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| Institution: | Xiao Fuying Liu Xixian Yang Zhenrong Department of Biology,You] iang Medical Collage for National Minorities,Bose,Guangxi,China |
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| Abstract: | Studies were made by using biotinglated Y chromosome specific 3.4kb DNA probe for chromosome in situ hybridization in a case of phenotypic anomalies and a small Y chromosome with conventional chromosome-banding techniques. The results showed the case didn't hybrid to 3.4 kb DNA but corresponded with ~3H-labeled probe for chromosome in situ hybridization. Southern blotting of the case revealed that the case lacked part of the commonly detected male-specific fragment. Based on the case clinical character, we suggested that the case was interstitial delection of Yq. |
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| Keywords: | biotinglated Y specific DNA probe Y chromosome chromosome in situ hybridization Southern blotting |
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