1例乳糖发酵鼠伤寒沙门菌腹泻病例株的表型和分子特征研究

目的 研究首次发现的乳糖发酵鼠伤寒沙门菌腹泻病例株的耐药型和分子型特征, 优化网络实验室检测方法。方法 网络参比实验室分别使用系统生化鉴定、血清分型鉴定、纸片扩散法和脉冲场凝胶电泳(PFGE)分型对菌株进行生化特征和血清型、耐药表型和分子型的复核鉴定。结果 菌株符合乳糖发酵的鼠伤寒沙门菌表型特征, 耐药型为多重耐药株, PFGE图谱经本地数据库比对属于新的分子型, 其遗传特征归属于本地的流行大克隆。结论 乳糖发酵的非伤寒沙门菌病例菌株的发现过程和鉴定对诊断和治疗感染性疾病、优化实验室检测流程、提升网络实验室的能力、阐述肠道菌遗传进化的生物多样性等方面具有多重意义。

Study on phenotype and molecular characteristics of lactose-fermenting Salmonella typhimurium isolated from one diarrhea case

Objective To understand the drug resistant phenotype and molecular characteristics of the first isoalted lactose-fermenting Salmonella typhimurium strain from one diarrhea case and optimize the detection. Methods Biochemistry identification, serotype identification, disk diffusion test and pulsed field gel electrophoresis (PFGE) were condcuted to identify the biochemical characteristics, serotype, drug resistant phenotype and molecular characteristics of the strain respectively in network reference laboratory. Results The phenotype characteristics of the strain were consistent with the phenotype characteristics of the Salmonella typhimurium, the drug resistant phenotype was multi drug resistance (MDR), the molecule type was identified as a new type through the comparison of PFGE patten of the strain with that in local databases and the genetic characteristics of the strain were consistent with local circulating clone. Conclusion The identification of lactose-fermenting Salmonella typhimurium is of great importance for the diagnosis and treatment of communicable diseases, improvement of network reference laboratory capcity and explaination of biodiversity in genetic evolution of intestinal bacterial flora.