2013年青海省德令哈地区蚊虫及蚊媒病毒调查

目的 调查青海省海西州德令哈地区蚊虫及蚊媒病毒的种类及分布。方法 在德令哈地区采集蚊虫标本, 蚊虫标本经实验室常规处理后使用C6/36、BHK-21和Vero细胞开展病毒分离及9种病毒特异性基因检测及分析。结果 2013年在德令哈地区共采集蚊虫6050只, 分别为黄背伊蚊和里海伊蚊, 其构成比分别为92.6%(5600/6050)和7.4%(450/6050)。所有蚊虫标本分为96批, 接种上述3种细胞, 这些蚊虫标本在BHK-21和Vero细胞上均未出现病变也未检测病毒基因阳性。但有32批蚊虫标本接种的C6/36细胞上清液和对应蚊虫标本研磨液中均检测到辽宁病毒(liaoningvirus, LNV), 其中黄背伊蚊27批, 里海伊蚊5批。病毒基因进化分析发现德令哈市分离的LNV与我国在新疆分离的LNV处在同一进化分支。结论 2013年青海省德令哈地区黄背伊蚊为优势蚊种, 黄背伊蚊和里海伊蚊标本中LNV感染率均较高, 为首次在当地发现LNV。

Investigation of mosquitoes and arboviruses in Delingha area in Qinghai, 2013

Objective To investigate the species and distributions of mosquitoes and arboviruses in Delinha of Qinghai province. Methods Mosquito samples were collected from Delinha. The virus isolation from these samples was conducted by using C6/36, BHK-21 and Vero cells, and the isolates were identified with molecular biological methods. Results A total of 6050 mosquito samples were collected from in 2013, including 5600 Aedes flavidorsalis (92.6%) and 450 Aedes caspius (7.4%). All the mosquitoes were divided into batches to be inoculated with C6/36, BHK-21 and Vero cells for virus isolation. No pathogenic changes and virus genes were detected in all the mosquito samples inoculated with BHK-21 and Vero cells, but Liaoning virus(LNV)was detected in 32 batches of mosquitoes inoculated with C6/36 cells, i.e. supernate and grinding fluid, including 27 batches of Aedes flavidorsalis and 5 batches of Aedes caspius (15.6%). Phylogenetic analysis revealed that the LNV isolated from Delingha was in the same evolutionary branch with LNV isolated in Xinjiang. Conclusion Aedes flavidorsalis was predominant mosquito specie in Delingha in 2013. The LNV infection rates were high in both Aedes flavidorsalis and Aedes caspius. This was the first time to detect LNV in Delingha.