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| 雷公藤多苷对大鼠局灶性脑缺血再灌注损伤的保护作用 | |
| 引用本文： | 张艳,贾丹辉,刘宗文,王金兰,张钦宪,李宏. 雷公藤多苷对大鼠局灶性脑缺血再灌注损伤的保护作用[J]. 中国新药与临床杂志, 2005, 24(11): 862-865 |
| 作者姓名： | 张艳 贾丹辉 刘宗文 王金兰 张钦宪 李宏 |
| 作者单位： | 1. 郑州大学基础医学院,药理学教研室,河南,郑州,450052 2. 郑州大学医学院,河南,郑州,450052 3. 郑州大学第二附属医院,神经内科,河南,郑州,450003 4. 郑州大学基础医学院,河南,郑州,450052 5. 河南大学医院,药剂科,河南,开封,475001 |
| 基金项目： | 河南省教育厅自然科学基础研究计划项目（97310010） |
| 摘 要： | 目的:观察雷公藤多苷(TwP)对局灶性脑缺血再灌注损伤大鼠的保护作用。方法:180只大鼠随机分为脑缺血再灌注组,TwP大、中、小剂量组,阳性对照组,假手术组。灌胃给药,采用大脑中动脉局灶性缺血再灌注模型,测定再灌注2 h缺血侧脑组织含水量、超氧化物歧化酶(SOD)、丙二醛含量及大脑皮层神经元细胞内[Ca2+]i水平。结果:与假手术组相比,脑缺血再灌注组脑组织丙二醛含量增加、SOD活性降低、含水量及[Ca2+]i显著升高(P<0.01);与脑缺血再灌注组相比,TwP大、中、小剂量组,阳性对照组脑组织丙二醛含量减少、SOD活性升高、[Ca2+]i显著下降(P<0.01或P<0.05),TwP大、中剂量组,阳性对照组脑组织含水量显著降低(P<0.01);TwP各剂量组间结果亦有显著差异(P<0.01或P<0.05)。结论:TwP能减轻大鼠急性脑缺血再灌注损伤引起的脑水肿、降低脑组织丙二醛含量,提高SOD活性,降低细胞内钙积累;且随着剂量增加,变化显著增强,提示雷公藤多苷对大鼠急性脑缺血再灌注损伤具有保护作用。 |
| 关 键 词： | 脑缺血 再灌注 丙二醛 超氧化物歧化酶 游离钙 雷公藤多苷 |
| 文章编号： | 1007-7669（2005）11-0862-04 |
| 收稿时间： | 2005-03-02 |
| 修稿时间： | 2005-03-022005-09-15 |
Protective effects of Tripterygium wilfordii polyglycosidum on focal cerebral ischemia/reperfusion of injured rats |
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| ZHANG Yan,JIA Dan-hui,LIU Zong-wen,WANG Jin-lan,ZHANG Qin-xian,LI Hong. Protective effects of Tripterygium wilfordii polyglycosidum on focal cerebral ischemia/reperfusion of injured rats[J]. Chinese Journal of New Drugs and Clinical Remedies, 2005, 24(11): 862-865 | |
| Authors: | ZHANG Yan JIA Dan-hui LIU Zong-wen WANG Jin-lan ZHANG Qin-xian LI Hong |
| Affiliation: | 1. Department of Pharmacology, College of Basic Medical Science ,Zhengzhou University, Zhengzhou HENAN 450052, China; 2. Medical Science College of Zhengzhou University, Zhengzhou HENAN 450052, China; 3. Department of Neurology, the Second Affilicated Hospital of Zhengzhou University, Zhengzhou HENAN 450003, China; 4. College of Basic Medical Science, Zhengzhou University, Zhengzhou HENAN 450052, China; 5. Department of Pharmacy, the Hosptial of He-nan University, Kaifeng HENAN 475001, China |
| Abstract: | AIM: To observe the protective effects of Tripterygium wilfordii polyglycosidum(TwP) on focal cerebral ischemia/reperfusion(I/R) areas of injured rats. METHODS: One hundred and eighty rats were randomly divided into I/R group,sham-operated group,TwP groups(including large,middle and small doses groups), nimodipine(Nim) group.I/R model was made by repeatedly inserting a nylon thread method to investigate the reperfusion through out the contents of brain water and malondialdehyde(MDA) activities of SOD in the brain tissues and the intracellular [Ca~(2 )]_i in the cerebral cortex.RESULTS: Compared with I/R group,TwP and nimodipine groups showed reduction of the contents of brain water,MDA and [Ca~(2 )]i and increase of the activities of SOD significantly(P<0.01 or P<0.05). CONCLUSION:TwP has a neural protection on the cerebral ischemia/reperfusion foci of the injured rats by reducing the content of brain water,MDA and [Ca~(2 )]_i and increasing the activities of SOD. |
| Keywords: | cerebral ischemia reperfusion malondialdegyde superoxide dismutase [Ca~(2 )]_i tripterygium wilfordii polyglycosidum |
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