小鼠双侧卵巢自体异位移植的形态学研究

对生后３０ｄ雌性小鼠进行双侧卵巢自体皮下移植，研究移植物的生长发育和分泌功能。取移植卵巢进行ＨＥ染色观察。卵巢外包较厚的结缔组织膜。卵泡坏死始于移植后第２ｄ，血细胞进入坏死腔内；第４ｄ皮质浅层见大量原始卵泡和少量窦前卵泡；第７ｄ卵巢生发上皮明显，皮质中见少量窦状卵泡、囊状卵泡和间质腺；第１４～２１ｄ坏死灶基本消失，并可见黄体样结构；第２８～７２ｄ卵巢中充满正在生长的各期卵泡和黄体，可见间质腺。动情周期于移植后（７．９±１．７）ｄ恢复。实验显示自体双侧异位移植卵巢能够存活、发育，并分泌性激素，维持性周期。

A Morphologic Study on the Bilaterally Autotransplanted Ovary in White Mouse

Two ovaries of the female mouse aged 30 postnatal days were autotransplanted subcutaneously in order to study the growth and secretion of the transplanted ovaries.The ovarian graft was surrounded by a thick layer of connective tissue.Follicle necrosis began on day 2.Blood cells infiltrated into the necrotic area.Four days after transplantation ,there were some primodial follicles and preantral follicles in the outer cortex of the ovary.On day 7,a few antral follicles, graffian follicles and interstitial-land-like tissue could be detected.On day 14 to 21, there were luteinic tissue in the graft.Necrosis disappeared.28 to 72 days after grafting, a lot of follicles in different stages of development, corpora luteas and interstitial glands could be found.The estrus cycle emerged 7.9±1.7 days after transplantation.This study indicates that bilaterally autotransplanted ovaries can grow and secrete sex hormones which maintain the estrus cycle.