葡萄籽原花青素对复发性结肠炎大鼠血清抗氧化能力及NO含量的影响

目的：观察葡萄籽原花青素（GSPE）对复发性结肠炎大鼠血清抗氧化能力及NO含量的影响,初步探讨葡萄籽原花青素治疗复发性结肠炎的作用机制。方法：直肠给予雄性Wistar大鼠80mg/kg2,4,6-三硝基苯磺酸（TNBS）/50%乙醇溶液复制结肠炎模型,在第16天时,用30mg/kgTNBS/50%乙醇溶液诱导结肠炎复发的模型。大鼠第二次致炎24h后,分别应用GSPE低、中、高剂量（100、200、400mg/kg）灌胃对其进行治疗,并以柳氮磺吡啶（SASP,500mg/kg）作为阳性对照。连续给药7d后处死所有大鼠,取结肠标本评价结肠湿重指数,生化法检测血清中髓过氧化物酶（MPO）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和一氧化氮合酶（iNOS）活力及丙二醛（MDA）、谷胱甘肽（GSH）和NO含量。结果：与模型对照组比较,GSPE各剂量组大鼠体重下降程度较轻（P〈0.05或P〈0.01）,结肠湿重指数明显降低（P〈0.05或P〈0.01） 大鼠血清中MPO和iNOS活力及MDA和NO含量均明显降低（P〈0.05或P〈0.01） 大鼠血清中SOD和GSH-Px活力及GSH含量明显升高（P〈0.05或P〈0.01）。结论：GSPE可能通过提高复发性结肠炎大鼠血清抗氧化能力,抑制NO生成,来减轻复发性结肠炎炎症反应。

Effects of proanthocyanidins from grape seeds on the antioxidation in the resistance to oxidation and NO comtents

AIM:To investigate the antioxidation of proanthocyanidins from grape seeds (GSPE)on 2,4,6-trinitrobenzene sulfonic acid (TNBS)twice-induced recurrent ulcerative colitis(UC)in rats and to probe into its underlying mechanism.METHODS:Recurrent colitis model was established in Wistar male rats by rectal administration of 80 mg/kg TNBS dissolved in 50%ethanol,and then the rats were second instilled with 30 mg/kg TNBS into the colon on the 16th day after the first induction UC.Rats were intragastrically administered different doses of GSPE(100,200,and 400 mg/kg)per day for 7 days after twice-induction of colitis by TNBS.Sulfasalazine(SASP,500 mg/kg)was used as a positive control drug.Rats were killed after GSPE treatment 7 days.The colon sample was extracted,and the colon weight/length ratio (mg/cm)was assessed.The activities of,myeloperoxidase(MPO),superoxide dismutase (SOD),glutathione peroxidase(GSH-Px),inducible nitric oxide synthase(iNOS),and the contents of malonyldialdehyde(MDA)and glutathione(GSH)and NO in serum were detected by biochemistry method.RESULTS:Compared with the model group,the rat body weight was decreased,and the colonic weight/length ratio was reduced in GSPE dosage group(P＜0.05 or P＜0.01);the activities of MPO and iNOS and the contents of MDA,NO in serum were significantly decreased(P＜0.05 or P＜0.01);but the activities of MPO,iNOS and the contents of MDA,NO were decreased(P＜0.05 or P＜0.01);the activities of SOD,GSH-Px,the contents of GSH in serum were increased(P＜0.05or P＜0.01).CoNCLUSION:GSPE can alleviate the inflammatory reactions in colitis through inhibiting oxygen free reaction-exerting antioxidation effects and decreasing the production of NO.