精氨酸与瘦素联合治疗急性胰腺炎的临床研究

目的 探讨精氨酸联合瘦素治疗大鼠急性胰腺炎的疗效.方法 Wister大鼠60只,随机分为假手术组(A组)、急性胰腺炎组(B组)、精氨酸治疗组(C组)、瘦素治疗组(D组)和联合治疗组(E组),每组12只;采用5%牛磺胆酸钠胰胆管逆行注入制作急性胰腺炎模型.每组大鼠均在腹腔注射治疗6 h后行腹主动脉采血、取胰腺标本;检测每组血清肿瘤坏死因子-α(TNF-α)的含量、淀粉酶;胰腺组织核因子-κB(NF-κB)活性,并对胰腺损伤进行病理评分.结果 A组TNF-α的含量、淀粉酶、NF-κB活性、病理评分依次为(1.206±0.446)μg/L、(1617.2±18.8)U/L、0.174±0.013、0.6±0.2;B组依次为(3.201±0.321)μg/L、(4643.8±303.4)U/L、0.397±0.025、7.8±1.3;C组依次为(1.845±0.404)μg/L、(3370.9±173.2)U/L、0.255±0.026、3.9±1.1;D组依次为(1.996±0.374)μg/L、(3693.7±208.0)U/L、0.274±0.062、4.2±1.2;E组依次为(1.440±0.287)μg/L、(2501.0±771.4)U/L、0.211±0.027、2.3±1.0.与A组相比,B组上述指标均增高明显(P均<0.05);与B组相比,C组和D组以上指标均降低(P均<0.05),而C组与D组间差异均无统计学意义(P均>0.05);E组较C组和D组的上述指标均明显降低(P均<0.05).且NF-κB与其余指标均呈正相关(P<0.01).结论 精氨酸和瘦素在治疗大鼠急性胰腺炎过程中疗效确切且两药联合治疗效果优于单一用药.

Study of arginine in combination with leptin in treatment of pancreatitis

Objective To investigate the effects of arginine combined with leptin in treatment of rats with a-cute pancreatitis. Methods Sixty Wister rats were randomly divided into sham operation group (group A), acute pancreatitis group (group B),arginine group (group C),leptin group (group D),arginine plus leptin group (group E),with 12 rats in each group. Acute panereatitis model was developed by retrograde injection of 5% sodium tauro-cholate into cholangiopancreatography in rats. After 6 hours, medicine were given by intraperitoneal injection, ab-dominal aortic blood and pancreatic specimens were taken for measurement of tumor necrosis factor-α(TNF-α), ser-um amylase,nuclear factor-κB (NF-κB) activity of pancreatic tissue, and pathological damage score of pancreatic tissue. Results TNF-α,Amylase,NF-κB activity, pathologic scores were(1.206±0.446)μg/L, (1617.2±18.8) U/L,0.174±0.013,0.6±0. 2 in group A;(3.201±0.321) μg/L, (4643.8±303.4) U/L,0.397±0. 025,7.8±1.3 in group B;(1.845±0.404) μg/L,(3370.9±173.2)U/L,0.255±0.026,3.9±1.1 in group C;(1.996±0.374) μg/L, (3693.7±208.0)U/L,0.274±0.062,4.2±1.2 in group D;(1.440±0.287)μg/L, (2501.0±771.4)U/L,0.211±0.027,2.3±1.0 in group E. Compared with group A, the indicators were significantly in-creased in group B (P < 0.05). Compared with group B, these indicators were reduced in group C and D (P <0.05). There was no significant difference between group C and group D. These indicators in group E were signifi-cantly lower than those in group C and D (P < 0.05). NF-κB in pancreatic tissue were positively correlated with other indicators(P <0.01). Conclusions In treatment of acme pancreatitis in rats, arginine combined with leptin has idenditfied effect, and the effect of combination therapy is better than that of separate medicine.