检测胃癌患者外周血中K-ras和p53基因突变的临床价值

目的 探讨检测胃癌患者外周血中K-ras和p53基因突变的临床意义.方法 应用聚合酶链反应一单链构象多态性分析(PCR-SSCP)法,对我院收治的73例胃癌患者外周血中的K-ras和p53基因突变进行检测,并与20例非肿瘤疾病患者作对熙.结果 73例胃癌患者中有7例外周血中检测出p53基因突变,阳性率为9.6%;5例检测出K-ras基因突变,阳性率为6.8%.对照组外周血中均未检出K一瑚和p53基因突变.有无肝转移的胃癌患者外周血p53和K-ras基因突变阳性率间差异均有统计学意义(P＜0.05).不同分化程度及临床分期的胃癌患者外周血p53和K-ras基因突变阳性率间差异无统计学意义(JP＞0.05).结论 PCR-SSCP法检测外周血中K-ras和p53基因DNA有助于胃癌肝转移的诊断.

Significance of p53 and K-ras Gene Mutation of Peripheral Blood in Diagnosis of Gastric Carcinoma

Objective To investigate clinical significance of p53 and K-ras mutation of peripheral blood in patients with gastric carcinoma.Methods Totally 73 patients with gastric carcinoma were chosen to detect gene mutation of p53 and K-ras with PCR-SSCP technology,and another 20 cases of non-tumor patients were enrolled as the controls.Results Among 73 patients,there were 7 who have p53 mutation in peripheral blood,with positivity rate of 9.6%,and 5 who have K-ras mutation,with a positivity rate of 6.8%.No p53 and K-ras mutation was found in peripheral blood of the controls.There was a significant difference in positivity rate of the p53 and K-ras mutation between the patients who have liver metastasis and who have not (P<0.05),however,there was no significant difference in positivity rate of the p53 and K-ras mutation between the patients with different cancer grades of differentiation and clinicopathologic stages (P>0.05).Conclusion Detection of gene mutation of cancer cell in peripheral blood by PCR-SSCP has a certain value to early diagnosis of hepatic micrometastasis in gastric carcinoma.