人参总皂苷中Rg1的吸收特性研究

目的：建立准确、灵敏、可靠的Rg1 HPLC-MS测定方法,研究总皂苷及单体给药后Rg1在小肠各段的吸收情况,考察是否存在分肠段吸收差异.方法：在不同肠段（十二指肠、空肠、回肠和结肠） 的在体肠袢中注入Rg1或含等量Rg1的总皂苷水溶液,在不同时间点取血样,提取后测定血内药物浓度,比较各个肠段对Rg1吸收的差异.同时比较单体Rg1和含等量Rg1的总皂苷水溶液灌胃后,大鼠体内Rg1的血药浓度经时过程.结果：总皂苷灌胃后,大鼠体内Rg1的血药浓度在 6 h 出现明显的双峰,而单体Rg1给药后没有出现双峰现象.不同肠段在体肠袢模型实验及灌胃实验结果表明,总皂苷给药后Rg1的吸收明显好于单体给药,总皂苷及单体给药后Rg1在不同肠段的吸收有明显差异,在十二指肠和回肠两个肠段无明显差异.结论：单体Rg1和总皂苷中Rg1在大鼠体内吸收存在明显差异,总皂苷中可能含有某种特殊物质可以在十二指肠和回肠促进Rg1的吸收.

Study on absorption of ginsenoside Rg1 using rat ansa intestinal model

AIM: To develop an accurate,sensitive and reliable HPLC-MS method for the determination of ginsenoside Rg1 in rat plasma,and to study the absorption characteristics of Rg1 at different intestinal sections(duodenum,ileumand,jejunum and colon) of rats.METHODS: Ginsenoside Rg1 or total ginsenoside which containing equal amount of Rg1 was injected into different intestinal sections(duodenum,ileum,jejunum and colon) of each rat.At the same time,Ginsenoside Rg1 or total ginsenoside was administrated to rats by intragastric gavage.Blood samples were collected according to preset timetable and Rg1 concentrations were determined.RESULTS: The bioavailability of Rg1 in rats was increased significantly when total ginsenoside was administrated compared with that of Rg1 given alone.The absorption profile of Rg1 was different in duodenum and ileum but similar in jejunum and colon.CONCLUSION: There might be some specific substance in total ginsenoside,which could improve the absorption of Rg1 in duodenum and ileum.