腰椎间盘的超微结构研究

本实验通过透射电镜对大鼠、家兔及人(胎儿及成人)腰椎间盘的观察,发现纤维环由纤维软骨组成。在640 A周期性的胶原原纤维之间,可见位于隐窝内的典型软骨细胞。这些细胞常随所在部位而异。靠近周边部位的细胞更接近成纤维细胞,细胞狭长,细胞膜的扇蛤样外形渐不明显,以至消失。靠内侧的细胞类似脊索细胞,细胞核固缩,细胞质内有浓集的糖原及脂滴。纤维环呈现细胞过渡的特点。纤维环内可见弹力纤维。髓核由包含细的原纤维、颗粒及特殊结构的疏松基质所组成。在各标本中,基质内均有脊索细胞出现。细胞可单独或成堆存在,细胞质内有众多的空泡,浓集的糖原颗粒及微丝。

ULTRASTRUCTURAL INVESTIGATION OF LUMBAR INTERVERTEBRAL DISC

The normal lumbar intervertebral discs of rat, rabbit and human (fetal and adult) were studied under transmission electron microscope. In all the specimens the following findings could be observed. The annulus fibrosus was composed of fibrocartilage. However, the cells in the lacunae of the fibrocartilage might show changes in form with the location of the lacunae. The cells in those lacunae on the periphery of the annulus became elongated and lost their scalloped cytoplasmic membrane, thus resembled fibroblasts. On the contrary, the cells in those lacunae in the central portion of the annulus revealed characteristics of the notochordal cells, with pyknosis of nucleus and aggregation of glycogen and fat droplets in the cytoplasm. Consequently the annulus fibrosus exhibited a transition of their cellular components. Elastic fibers could be found in the annulus. Nucleus pulposus contained notochordal cells in a loose matrix. The notochordal cells might exist singly or in clusters and in their cytoplasm there were many vacuoles and aggregates of glycogen and microfilaments.