微囊藻毒素LR对SD大鼠的短期毒效应研究

目的：研究和探讨微囊藻毒素（MCLR）对动物的短期毒效应作用。方法：SD大鼠经腹腔注射不同剂量的MCLR,分别于注射的1、7d和停药后第7天（即第14天）采目力大鼠的血清和肝、肾、心等组织标本,经酶学及病理学检验,观察其损伤效应。结果：122цg/kg剂量组,注射后24h可观察到大鼠心肌细胞肌浆溶解,核变性固缩,肌原纤维局部坏死;血清中天冬氨酸转氨酶(AST)、乳酸脱氢酶（LDH）和磷酸肌酸激酶（CPK）与其他组相比显著升高;肾细胞变性,血清中肌酐（BCr）和尿素氮（BUN）亦显著升高;同时肝细胞片状出血、坏死,注射剂量为50цg/kg和25цg/kg时,仍出现肝细胞重度和轻度颗粒变性。血清中丙氨酸转氨酶（ALT）、碱性磷酸酶（LDH）和AST显著上升。对照组大鼠的肝、肾、心等脏器均正常。结论：MCLR可引起SD大鼠肝、肾、心等脏器的短期毒效应,且随着剂量的增加,损伤效应加重。

The acute toxic effects of microcystin LR in SD rats

OBJECTIVE: To assess Microcystin LR (MCLR)-induced acute toxic effects in male Sprague-Dawley rats. METHODS: The rats were injected with MCLR intraperitoneally in different doses for different days.The organs and serum with rats were collected at 1 and 7 days after injection, and 7 days after the final injection (total 14 days). Pathological and enzymatic changes were observed. RESULTS: The rats injected with 122 microg/kg MCLR showed myocardial cells damage including pyknosis, plasma dissolve and myofibrilla (pls check with dictionary) necrosis in the heart muscles after 24 hours. At the same time, the activities of serum glutamate-oxaloacetate transaminase (GOT), lactate dehydrogenase (LDH) and creatine phosphonase (CPK) were higher than these in the other groups (P < 0.01). The kidney was also damaged, kidney cell degeneration, and the increase of blood creatine (BCr) and blood urea nitrogen (BUN) were also seen. In liver pathological study, liver cell hemorrhage, degeneration and/or necrosis was observed. In serum the activities of glutamate-pyruvate transaminase (GPT), alkaline phosphatase (LDH) and GOT were higher than these in the other groups (P < 0.01). CONCLUSION: These results suggested that MCLR can injure the heart, kidney and the liver in SD rats, and there is a dose-response relationship between MCLR and the toxic effect.