复方黄连胶囊对早期糖尿病肾病大鼠肾组织VEGF蛋白和mRNA表达的影响研究

目的:探讨复方黄连胶囊(CRCC)对早期糖尿病肾病大鼠肾组织VEGF蛋白和mRNA表达的影响。方法:腹腔一次性注射链脲佐菌素复制早期糖尿病肾病大鼠模型,分为正常对照、模型、消渴丸(0.8g·kg-1)及CRCC低、高剂量(生药含量分别为2.18、4.36g·kg-1)组,灌胃给药每日1次,8周后检测大鼠空腹血糖(FBG)、肌酐、尿素氮;放免分析检测血清胰岛素(Ins)、α1微球蛋白(α1-MG)、β2微球蛋白(β2-MG)水平;免疫组化技术和逆转录聚合酶链反应(RT-PCR)检测肾组织VEGF蛋白和mR-NA表达。结果:与模型组比较,CRCC给药组大鼠FBG、α1-MG、β2-MG显著降低(P<0.01),Ins显著升高(P<0.01),VEGF蛋白和mRNA的表达减弱(P<0.05)。结论:CRCC可显著降低早期糖尿病肾病大鼠肾组织VEGF蛋白和mRNA的过度表达,改善肾功能,延缓糖尿病肾病发展。

Effects of Compound Rhizoma Coptidis Capsule on the Expressions of Vascular Endothelial Growth Factor and mRNA in Renal Tissue of Rats with Early Diabetic Nephropathy

OBJECTIVE： To explore the effects of compound Rhizoma coptidis capsule on the expressions of vascular endothelial growth factor （VEGF） and mRNA in the kidney of rats with early diabetic nephropathy. METHODS： The rats were intraperitoneally administered with streptozotocin to establish early diabetic nephropathy model. Then the rats were randomly divided into five groups： normal control group, diabetic nephropathy model group, Xiaoke Pill （0.8 g·kg^-1） group, CRCC-treated groups （2.18 g ·kg ^-1 and 4.36 g·kg^-1） . The animals were intragastrically administered with corresponding drugs once daily. After 8 weeks, the fasting blood glucose, blood urea nitrogen and creatinine werc measured and serum levels of insulin （Ins）, a1-MG, β2 MG were determined by radioimmunoassay. Immunohistochemistry and RT PCR were used to detect the expressions of VEGF and mRNA in the renal tissue of rats. RESULTS： In CRCC treated groups, serum levels of FBG, a1- MG,β2-MG, BUN and Scr were markedly decreased （P 〈 0.01）, while the insulin level increased markedly（P 〈 0.01）, and the expressions of VEGF and mRNA in renal glomeruli and tubuli were decreased（P 〈0.05） .CONCLUSION： CRCC can obviously improve renal function and decrease the overexpression of VEGF and mRNA in kidney tissue, and delay the progression of diabetic nephropathy in diabetic nephropathy model rats.