| RNA干扰人端粒酶hTR基因对HeLa细胞增殖的影响 |
| |
| 引用本文： | 朱俊峰,袁广卿,黄小荣,骆晓枫,周俊宜. RNA干扰人端粒酶hTR基因对HeLa细胞增殖的影响[J]. 实用医学杂志, 2010, 26(4). DOI: 10.3969/j.issn.1006-5725.2010.04.010 |
| |
| 作者姓名： | 朱俊峰 袁广卿 黄小荣 骆晓枫 周俊宜 |
| |
| 作者单位： | 1. 中山大学中山医学院生物化学与分子生物学教研室,广州市,510080
2. 中山大学基础医学实验教学中心 |
| |
| 基金项目： | 广东省自然科学基金重点项目，广东省科技计划项目 |
| |
| 摘 要： | 目的:探讨RNA干扰抑制人端粒酶RNA组分human telomerase RNA component,hTR)的表达时宫颈癌细胞(HeLa细胞)增殖的影响.方法:hTR基因的RNA干扰表达重组体用脂质体介导方法稳定转染HeLa细胞.RTPCR法检测hTR的mRNA表达水平,TRAP-PCR法检测端粒酶活性,MTT法检测细胞增殖.结果:RT-PCR结果表明,稳定转染RNA干扰表达重组体的细胞株,其hTR基因的mRNA抑制率较无转染对照组下降了(59.7±3.3)%,较转染空载体组下降了(56.3±4.4)%;实验组细胞端粒酶活性下降,生长速度明显减慢.结论:利用RNA干扰技术抑制hTR基因在HeLa细胞的表达能抑制该细胞端粒酶活性和增殖活性.
|
| 关 键 词： | RNA干扰 HeLa细胞 人端粒酶RNA亚基 小发夹RNA |
Effects of inhibition of human telomerase RNA by RNA interference on proliferation of HeLa cells |
| |
| ZHU Jun-feng,YUAN Guang-qing,HUANG Xiao-rong,LUO Xiao-feng,ZHOU Jun-yi. Effects of inhibition of human telomerase RNA by RNA interference on proliferation of HeLa cells[J]. The Journal of Practical Medicine, 2010, 26(4). DOI: 10.3969/j.issn.1006-5725.2010.04.010 |
| |
| Authors: | ZHU Jun-feng YUAN Guang-qing HUANG Xiao-rong LUO Xiao-feng ZHOU Jun-yi |
| |
| Affiliation: | ZHU Jun-feng,YUAN Guang-qing,HUANG Xiao-rong,LUO Xiao-feng,ZHOU Jun-yi. Department of Biochemistry , Molecular Biology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,510080,China |
| |
| Abstract: | Objective To investigate the effect of inhibiting hTR expression by RNA interference on the proliferation of HeLa cell. Methods A combination plasmids expressing shRNA specific for human telomerase RNA were transfected by Lipofectamine into HeLa cells. The inhibitory of hTR mRNA level was assayed by RT-PCR. The activity of telomerase was tested by TRAP-EB staining. The proliferation activity of HeLa cells was determined using methyl thiazolyl tetrazolium (MTT). Results As detected by RT-PCR,mRNA level of hT... |
| |
| Keywords: | RNAi HeLa cell Telomerase RNA shRNA |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
