P物质纳米缓释微球对人牙周膜成纤维细胞增殖作用的实验研究

目的：探讨P物质（SP）纳米缓释微球生物活性保存情况及其对于人牙周膜成纤维细胞的增殖作用。方法：分别将P物质和P物质-聚乳酸-聚乙醇酸共聚物（PLGA）纳米缓释微球加入人牙周膜成纤维细胞的培养液中，采用细胞计数法和四甲基偶氮唑盐微量反应比色法（MTT法）观察细胞增殖情况。结果：培养1d后，对照组、P物质组和P物质纳米缓释微球组的人牙周膜成纤维细胞数量和A值差异均无显著性意义（P〉0．05）；2d、3d时，P物质组的人牙周膜成纤维细胞数量和A值高于其余两组，差异有显著性意义（P〈0．05）：4d后，各组人牙周膜成纤维细胞数量和A值依次为P物质纳米缓释微球组〉P物质组〉空白对照组，P物质纳米缓释微球组和P物质组间差异无显著性意义（尸〉o．05）：培养6d、8d后，人牙周膜成纤维细胞数量和A值P物质纳米缓释微球组明显多于P物质组，组间差异有显著性意义（P〈0．05）。结论：SP-PLGA纳米微球通过持续释放活性SP，可在较长时间内促进牙周膜成纤维细胞的增殖。

Effect of controlled realease substance P-PLGA nanoparticles on cultured human periodontal ligament fibroblasts

Objective:To investigate the bioactivities of controlled release SP-PLGA nanoparticles and their effects on cultured human periodontal ligament fibroblasts.Method:The fifth cultured human periodontal ligament fibroblasts were divided into three groups according to the different ingredients being added to the DMEM culture medium,ie,control group,SP group and SP-PLGA group.The proliferation of the cultured cells was measured with cell counting method and MTT method.Result:The invitro cellular study showed no significant difference in the cell number and cell viability of three groups 1 day after plate culture.The cell number and cell viability in the SP group were more than those in other two groups 2,3 days after plate culture with significant difference,but after 4 days there was no diference between the SP group and the SP-PLGA group.The cell number and cell viability those in the SP-PLGA group were more than those in the SP group 6 and 8 days after plate culture with significant difference.Conclusion:It suggested SP-PLGA can promote the proliferation of human periodontal ligament fibroblasts through a long period of controlled release of SP.