Myofibrillar misalignment correlated to triad disappearance of mdx mouse gastrocnemius muscle probed by SHG microscopy |
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| Authors: | Denis Rouède Pascal Coumailleau Emmanuel Schaub Jean-Jacques Bellanger Mireille Blanchard-Desce Fran?ois Tiaho |
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| Affiliation: | 1.IPR, CNRS, UMR-CNRS UR1- 6251, Université de Rennes1, Campus de Beaulieu, Rennes, F-35000, France;2.IRSET, INSERM, U1085, Université de Rennes1, Campus de Beaulieu, Rennes, F-35000, France;3.LTSI, INSERM, U1099, Université de Rennes1, Rennes, F-35000, France;4.ISM, CNRS, UMR-CNRS 5255, Université de Bordeaux 1, Talence, F-33405, France |
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| Abstract: | We show that the canonical single frequency sarcomeric SHG intensity pattern (SHG-IP) of control muscles is converted to double frequency sarcomeric SHG-IP in preserved mdx mouse gastrocnemius muscles in the vicinity of necrotic fibers. These double frequency sarcomeric SHG-IPs are often spatially correlated to double frequency sarcomeric two-photon excitation fluorescence (TPEF) emitted from Z-line and I-bands and to one centered spot SHG angular intensity pattern (SHG-AIP) suggesting that these patterns are signature of myofibrillar misalignement. This latter is confirmed with transmission electron microscopy (TEM). Moreover, a good spatial correlation between SHG signature of myofibrillar misalignment and triad reduction is established. Theoretical simulation of sarcomeric SHG-IP is used to demonstrate the correlation between change of SHG-IP and -AIP and myofibrillar misalignment. The extreme sensitivity of SHG microscopy to reveal the submicrometric organization of A-band thick filaments is highlighted. This report is a first step toward future studies aimed at establishing live SHG signature of myofibrillar misalignment involving excitation contraction defects due to muscle damage and disease.OCIS codes: (180.4315) Nonlinear microscopy, (190.4160) Multiharmonic generation, (170.3880) Medical and biological imaging |
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