异种小血管支架模型的建立

目的　建立异种小血管支架模型。　方法　取34只Wistar大鼠,切取每只鼠尾动脉12 .0 8±1.6 9cm,经脱细胞制作成血管支架,常规制备电镜样本后观察。将未种植血管壁细胞的血管支架移植于5只日本大耳白兔耳中央动脉断端间,大体观察2 4 h。　结果　Wistar大鼠尾动脉近侧断端外径为0 .74±0 .0 8mm,远侧0 .5 5±0 .0 8mm,属于小血管。脱细胞的小血管支架壁薄、色白,呈半透明状,柔软,管腔塌陷,可折叠;主要由纤维组织构成,且内膜呈网络样密集排列。小血管支架移植于日本大耳白兔耳中央动脉后即刻通畅良好,2 4 h内可触及血管搏动,饱满,未见血液外漏。　结论　异种脱细胞处理的小血管支架以纤维组织构成为主;异种小血管支架移植后在2 4 h内能够承受动脉血冲击力;移植的血管以套叠式吻合法为宜。

CONSTRUCTION OF THE SCAFFOLD MODEL FROM HETEROGENEOUS SMALL BLOOD VESSELS

OBJECTIVE: To establish a scaffold model from heterogeneous small blood vessels. METHODS: Caudal arteries from 34 Wistar rats (average length 12.08 +/- 1.69 cm) were made into acellular blood vessel scaffolds. Some scaffolds were observed by electron microscope, and others were transplanted to the cut ends of ear central arteries of male Japanese big ear white rabbits. RESULTS: Average external diameter was 0.74 +/- 0.08 mm in proximal, and 0.55 +/- 0.08 mm in distal end of rat caudal arteries. The small blood vessel scaffolds had shin wall which was white and soft, composed of fibrous tissues without cells. On the intima surface the fibrous tissues were arrayed densely in a grid-like pattern. After transplantation, the blood flow was reserved, and kept flowing freely in 24 hours. The pulsation of the transplanted artery was accessible and no blood leakage was found. CONCLUSION: The natural scaffolds are composed of fibrous tissues, and can sustain the artery pulse pressure for 24 hours. It is better to suture the blood vessels by sleeve anastomosis.