超声造影定量分析技术评估大鼠肝纤维化程度的初步研究

目的将超声造影定量分析技术应用于大鼠肝纤维化模型，筛选相关的定量参数评估肝纤维化程度。方法对92例硫代乙酰胺（TAA）诱导的肝纤维化雄性SD大鼠自第3周起分批进行超声造影检查．使用定量分析软件SW—UCS-1对造影图像进行定量分析，得出各项定量参数，并与病理结果相比较。结果大鼠肝纤维化病理分级如下：S0 11例，S1 14例，S2 30例，S3 27例,S4 10例。超声造影定量参数中与纤维化程度有相关性的参数有：强度增量（r=-0．508，P〈0．001）、峰值减半速率（r=0．448，P〈0．001）、峰值强度（r=-．663，P〈0．001）、曲线下面积（r=-0．773，P〈0．001）及肝动静脉渡越时间（r=-0．642，P〈0．001）。结论超声造影定量分析技术可间接反映大鼠模型的肝纤维化程度。

Quantitative assessment with contrast-enhanced ultrasonography in a rat model of liver fibrosis

Objective Contrast-enhanced ultrasonography （CEUS） was used in a rat model with liver fibrosis in order to investigate quantitative parameters correlated with fibrosis grades. Methods Ninety-two male Sprague-Dawley rats were induced as liver fibrosis models by thioacetamide （TAA）. Results The fibrosis stage on pathology was as the following： S0=11, S1=14, S2=30, S3=27, and S4=10. There were statistical correlations between fibrosis stage and al （r=-0.508, P〈0.001 ）, PI （r=-0.663, P〈0.001）, AUC （r=-0.773, P〈0.001）, and transit time （r=-0.642, P〈0.001） from ROI of hepatic parenchyma. Conclusion The quantitative parameters on the time-intensity curve of dynamic CEUS images reflect the hepatic fibrosis stage in the rat model.