布渣叶保护急性心肌缺血损伤有效部位化学成分研究

目的 对布渣叶Microcos paniculata保护异丙肾上腺素致急性心肌缺血损伤有效部位化学成分进行研究.方法 采用硅胶、Sephadex LH-20柱色谱以及制备液相色谱等方法进行分离纯化,根据其波谱数据鉴定结构.结果 从布渣叶保护急性心肌缺血活性部位分离得到11个化合物,分别鉴定为牡荆苷(1)、异牡荆苷(2)、异鼠李素(3)、山柰酚(4)、槲皮素(5)、5, 6, 7, 8, 4''-五甲氧基黄酮(6)、5, 6, 7, 8, 3'', 4''-六甲氧基黄酮(7)、香草酸(8)、咖啡酸(9)、阿魏酸(10)、芥酸酰胺(11).结论 化合物6、7、9、11均为首次从该属植物中分离得到,其中化合物11为首次从该属植物中分离得到的脂肪酰胺类化合物,化合物6、7为首次从该属植物中分离得到的多甲氧基黄酮类化合物.

Chemical constituents from effective fraction of leaves of Microcos paniculata with protection on acute myocardial ischemia injury

Objective To study the chemical constituents from the effective fraction of the leaves of Microcos paniculata, which can protect acute myocardial ischemia injury induced by isoprenaline. Methods The chemical constituents were isolated and purified by combined using chromatography on silica gel, and Sephadex LH-20 column, and preparative HPLC. The structures of the isolated compounds were elucidated by NMR and MS spectral data. Results Eleven compounds were isolated from the effectively protective fraction with the protection on acute myocardial ischemia injury and identified as vitexin (1), isovitexin (2), isorhamnetin (3), kaempferol (4), quercetin (5), 5, 6, 7, 8, 4''-pentamethoxyflavone (6), nobiletin (7), vanillic acid (8), caffeic acid (9), ferulic acid (10), and erucicamide (11). Conclusion Compounds 6, 7, 9 and 11 are isolated from the plants in Microcos Linn. for the first time. Compound 11 is the firstly reported fatty acid amide and compounds 6 and 7 are the firstly reported polymethoxylated flavonoids with the obvious anti-inflammatory activity in this genus.