| rhsBLyS对B淋巴细胞增殖的影响 |
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| 引用本文： | 刘晓宇,张双全,王亚辉,杜秋丽. rhsBLyS对B淋巴细胞增殖的影响[J]. 免疫学杂志, 2005, 21(6): 525-527,531 |
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| 作者姓名： | 刘晓宇 张双全 王亚辉 杜秋丽 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,南京,210097;南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,南京,210097;南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,南京,210097;南京师范大学生命科学学院,江苏省分子医学生物技术重点实验室,南京,210097 |
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| 基金项目： | 南京师范大学科技创新基金资助项目(184070H80901) |
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| 摘 要： | 目的测定可溶性重组人B淋巴细胞刺激因子(rhsBLyS)对B细胞的促增殖活性。方法通过FluroBeadsB荧光磁珠法体外分离并培养人B淋巴细胞,以CD20的特异性抗体标记之,借助流式细胞术检测。结果rhsBLyS非融合蛋白在引发剂AntiIgM的协同作用下表现出显著刺激B淋巴细胞增殖的活性。结论流式细胞术检测简便高效,在验证样品活性的同时为进一步开展工作奠定了基础。
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| 关 键 词： | 流式细胞术 rhsBLyS B淋巴细胞 |
| 文章编号： | 1000-8861（2005）06-0525-04 |
| 收稿时间： | 2004-06-21 |
| 修稿时间： | 2004-06-212004-10-08 |
Effects of rhsBLyS on proliferation of B lymphocyte |
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| LIU Xiao-yu,ZHANG Shuang-quan,WANG Ya-hui,DU Qiu-li. Effects of rhsBLyS on proliferation of B lymphocyte[J]. Immunological Journal, 2005, 21(6): 525-527,531 |
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| Authors: | LIU Xiao-yu ZHANG Shuang-quan WANG Ya-hui DU Qiu-li |
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| Affiliation: | Jiangsu province key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing 210097, China |
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| Abstract: | Objective To investigate the biological activity of recombinant human soluble B lymphocyte stimulator (rhsBLyS). Me- thods B lymphocytes were separated from whole blood of healthy people by FluroBeads-B, and then cultured with rhsBLyS. The biological ac- tivity of rhsBLyS was detected by flow cytometry (FACS) with PE-CD20 molecules directly staining. Results The results shown that the protein could improve the B cells proliferation under synergistic effect of Anti-IgM. The numbers of B cells treated by the samples was significantly increased compared with those of the normal control group. Conclusion FACS is convenient and sufficient method, which provides a bases for further research. |
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| Keywords: | FACS rhsBLyS B lymphocyte |
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