丹参联合放疗抑制小鼠肺癌肿瘤血管生成的实验研究

目的:探讨丹参联合放疗对小鼠Lewis肺癌肿瘤组织微血管密度(MVD)的影响。方法:建立Lewis肺癌荷瘤鼠模型后,随机分为模型组、丹参低剂量组(丹低组)、丹参高剂量组(丹高组)、放疗组、丹参低剂量+放疗组(联合1组)、丹参高剂量+放疗组(联合2组),每组各10只小鼠。各组于接种第2日起每天腹腔注射给药,连续12天,放疗组、联合1组、联合2组于接种第7日起给予钴-60照射肿瘤局部,放疗剂量2 Gy/d,连续5天。末次给药24h后处死动物,剥离肿瘤组织称重,计算抑瘤率;免疫组化法检测肿瘤组织微血管密度(MVD)。结果:联合1组微血管密度较放疗组和丹低组明显降低(P<0.05)。结论丹参联合放疗对Lewis肺癌小鼠肿瘤血管生成有明显的抑制作用。

To Study on Anti-angiogenesis in Mice with Lewis Lung Carcinoma by Danshen Combined with Radiotherapy

Objective:To investigate generating impacts through using herb Danshen combined with radiotherapy.Methods:Holland Lewis lung carcinoma mice were taken as the model and randomly divided into six groups,i.e.control group,low doses of Danshen group,high doses of Danshen group,radiotherapy group,low dose of Danshen and radiotherapy group,high doses of Danshen and radiotherapy group,there were 10 mice in each group.Each group was inoculated by intraperitoneal and injected Danshen Injection since the second day of inoculation for 12 days.Radiotherapy group,low dose of Danshen and radiotherapy group,high doses of Danshen and radiotherapy group were irradiated with cobalt-60,2Gy/day for 5 days since 7th of inoculation,and stripped and weighing the tumors to calculate the inhibiting tumor rate,detected tumor microvessel density(MVD) with immunohistochemistry in vivo.Results:Microvessel density of Danshen of low dose combined with radiotherapy compared with radiotherapy and low dose of Danshen group was significantly reduced(P<0.05).Conclusion:Danshen combined with radiotherapy has obvious inhibited function on the angiogenesis of mice with Lewis Lung cancer.