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HPLC测定不同产地连翘中芦丁的含量
引用本文:娄政刚,刘瑞,张振秋.HPLC测定不同产地连翘中芦丁的含量[J].辽宁中医杂志,2012(2):329-330.
作者姓名:娄政刚  刘瑞  张振秋
作者单位:抚顺市卫生学校;辽宁中医药大学药学院
摘    要:目的:建立了连翘中芦丁的HPLC测定方法。方法:采用反相高效液相色谱法,Agilent Eclipse XDB-C18(4.6×250mm,5μm)色谱柱;流动相:乙腈-水(23∶77);检测波长:257nm;柱温:30℃;流速:1.0mL.min-1;进样量:20μL。结果:不同产地连翘中芦丁的含量差异显著。结论:此方法可用于检测连翘中芦丁的含量,为连翘的质量评价提供依据。

关 键 词:HPLC  连翘  芦丁  含量测定

Determination of Rutin in Forsythia Suspensa From Different Habitats by HPLC
LOU Zheng-Gang,Liu Rui,ZHANG Zhen-Qiu.Determination of Rutin in Forsythia Suspensa From Different Habitats by HPLC[J].Liaoning Journal of Traditional Chinese Medicine,2012(2):329-330.
Authors:LOU Zheng-Gang  Liu Rui  ZHANG Zhen-Qiu
Institution:1.Fushun Medical School,Fushun 113006,Liaoning,China; 2.College of Pharmacy,Liaoning Univesity of Traditional Chinese Medicine,Dalian 116600,Liaoning,China)
Abstract:Objective:The HPLC method was developed to determine Rutin in Forsythia suspensa.Methods:Use RP-HPLC and Agilent Eclipse XDB-C18(4.6×250 mm,5μm)columns.Mobile phase:acetonitrile-water solution.The detection wavelength:257nm;column temperature:30 ℃;velocity: 1.0 mL·min-1;the injection volume:20μL.Results:The level of Rutin in Forsythia suspensa from different commodities is significantly different.Conclusion:The method can be used for the determination of Rutin in Forsythia suspensa,which provides a scientific basis for quality assessment to Forsythia suspensa.
Keywords:HPLC  Forsythia suspensa  Rutin  determination
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