| 高效液相色谱法测定大鼠脑微渗析液中氟西汀的浓度 |
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| 引用本文： | 李迎春,姚国灿,郭兴杰.高效液相色谱法测定大鼠脑微渗析液中氟西汀的浓度[J].中国药房,2006,17(5):341-343. |
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| 作者姓名： | 李迎春 姚国灿 郭兴杰 |
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| 作者单位： | 1. 大连市第五人民医院药剂科,大连市,116023
2. 沈阳药科大学药学院,沈阳市,110016 |
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| 摘 要： | 目的建立以高效液相色谱法测定大鼠脑微渗析液中氟西汀浓度的方法。方法用正己烷提取氟西汀,经NBD-COCl衍生化,流动相为乙腈-水(50∶50),流速为1.2ml/min,柱温35℃,于ODS色谱柱分离,荧光检测波长λex=478nm、λem=537nm。结果大鼠脑微渗析液中氟西汀检测浓度线性范围为10~100nmol/L(r=0.9996),定量限为10nmol/L。结论该法专属性强,灵敏度和准确度高,适合生物样品中氟西汀的测定。
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| 关 键 词： | 氟西汀 高效液相色谱法 微渗析 |
| 文章编号： | 1001-0408(2006)05-0341-03 |
| 收稿时间： | 08 22 2005 12:00AM |
| 修稿时间： | 11 11 2005 12:00AM |
Assaying of Fluoxetine in Microdialysate in Rat's Brain by HPLC |
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| LI Yingchun,YAO Guocan,GUO Xingjie.Assaying of Fluoxetine in Microdialysate in Rat''''s Brain by HPLC[J].China Pharmacy,2006,17(5):341-343. |
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| Authors: | LI Yingchun YAO Guocan GUO Xingjie |
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| Abstract: | OBJECTIVE: To establish a HPLC method for the assaying of fluoxetine in microdialysate in rat's brain by HPLC. METHODS: Fluoxetine, which was extracted with N-hexane, was separated on ODS chromatographic column after derivatization by NBD-COCl,acetonitrile-water (50∶50) was the mobile phase with a flow rate of 1.2ml/min, column temperature of 35℃,fluorescence detection wave length λex at 478nm and λem at 537nm. RESULTS: The linear range for fluoxetine in microdialysate in rats' brain was 10～100 nmol/L(r=0.9 996), and the quantitation limit was 10 nmol/L. CONCLUSIONS: The method is specific, sensitive and accurate,it is suitable for the assaying of fluoxetine in biological specimen. |
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| Keywords: | Fluoxetine HPLC Microdialysis |
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