GABA受体基因转染对癫痫大鼠皮层脑电的影响

目的探讨在下丘脑乳头体上核内转染γ-氨基丁酸(GABA)受体基因后对海人藻酸(KA)致痫大鼠皮层脑电产生的影响,从而为难治性癫痫的治疗开辟新的途径。方法在右侧杏仁核内注射KA制备癫痫动物模型作对照,GABA基因转染组则利用仙台病毒(HVJ)-脂质体转染法预先在下丘脑的乳头体上核内转染被脂质体包被的GABA受体基因,48h后在杏仁核内注射KA,两组大鼠分别进行皮层脑电测定。结果KA致痫组大鼠脑电在尖波的背景上出现持续性放电,GABA转基因组大鼠则以散发的尖波、棘波以及阵发性放电为主,出现持续性放电的时间明显缩短。结论下丘脑内转染GABA受体基因后可以抑制癫痫发作的程度。

Effect of GABA receptor gene transfection on the cortical electroencephalogram of epileptic rats

Objective To investigate the changes of epileptic rats induced by Kainic acid (KA) after gene transfection of GABA receptors in hypothalamic supramammillary,and explore new therapeutic pathway for intractable epilepsy.Methods The rats were divided into two groups,the KA group for control group and the GABA gene transfection group.Epileptic models were built by injecting KA into the amygdala in control group.In GABA gene transfection group,GABA receptors gene were transfected into the supramammillary of hypothalamus by HVJ-liposome and then KA was injected into the amygdala after 48 hours.The cortical electroencephalogram of rats in both groups were determined respectively.Result In KA group,the maintained discharge was found in the background of sharp wave in electroencephalogram.And in GABA gene transfection group,time of maintained discharge was shorter and sharp wave,spike wave and paroxysma discharge was the main waves.Conclusion Seizure can be partly inhibited by gene transfection of GABA receptors in hypothalamic supramammillary.